Analysis of a candidate protein complex for the receptor of the maturation-inducing hormone of starfish oocytes

成熟诱导激素(MIH)诱导停滞在第一次减数分裂前期的动物卵母细胞成熟。海星MIH为1-甲基腺嘌呤(1-Meade)，在所有动物中首次被鉴定为MIH。尽管有人尝试鉴定1-Meade的受体，但目前仅发现它是一种G蛋白偶联受体，其分子仍不清楚。我尝试使用一种新材料--高性能亲和珠子(FG珠子或Handa珠子)进行受体识别。有三种蛋白质被鉴定为结合蛋白。这些蛋白质由与rendezvin基因同源的cdna编码。海星Rendezvin有两个Furin蛋白酶位点，Furin可以处理它们。由于海星rendezvin没有gpr的7个跨膜结构域，它可能与某些gpr结合。克隆了海星rendezvin结合伙伴的候选基因gpr。它的一部分片段与rendezvin结合。我提出了1-甲基腺嘌呤受体作为雷地平的一种独特的蛋白质复合体和一种新的GPCR。

Maturation-inducing hormone (MIH) induces maturation of animal oocytes arrested at pro-phase of first meiosis. Starfish MIH is 1-methyladenine (1-MeAde), which is first identified MIH in all animal. Even though there are attempts on identification of the receptor of 1-MeAde, it is only revealed as a G-protein coupled receptor (GPCR) and its molecule is still unclear. I tried the receptor identification using a novel material, high-performance affinity beads (FG-beads or Handa beads). Three proteins are identified as binding proteins. These proteins are coded by a cDNA homologous to the rendezvin gene. Starfish rendezvin has two furin protease sites and furin can process them. Since starfish rendezvin has no 7 transmembrane domain of GPCR, it might bind to some GPCR. A candidate GPCR is cloned for the binding partner of starfish rendezvin. A partial fragment of it binds to rendezvin. I propose 1-methyladenine receptor as a unique protein complex of rendezvin and a novel GPCR.