Reproduction barrier in interploidy crosses in Vitis

研究了葡萄倍性间和倍性内杂交后的种子形成。以欧洲葡萄品种蔷薇（V. vinifera）和砧木品种5BB （V. riparia x V. berlandieri）为材料，经秋水仙碱处理，将蔷薇与5BB杂交。花后30天，不同杂交的胚乳细胞有差异。这一阶段胚乳中蛋白体的积累与种子成熟后的萌发能力有关。对发育中的种子进行RNA-Seq分析。胁迫相关基因在倍性杂交中表达量较高。

Seed formation after inter- and intra- ploidy crosses were investigated in Vitis. Crosses were made between a European grape cultivar Rosaki (V. vinifera), a rootstock cultivar 5BB (V. riparia x V. berlandieri), and chromosome doubled clones produced from Rosaki and 5BB by colochicine treatment. Differences in endosperm cells were observed among crosses 30 days after flowering. Accumulation of protein bodies in endosperm at this stage correlated with the seed germination ability after seed maturation. RNA-Seq analysis was performed with developing seeds. Stress related gene expressions were high in inter-ploidy crosses.