Development of IgE mimotope-antibody fusion derivatives specific for sensitized allergens

屋尘过敏是一个严重的社会问题，建立其可治愈的治疗方法在我国也是一个紧迫的问题。本研究的目的是开发产生致敏变应原特异性IgE模拟表位-人免疫球蛋白抗体恒定区(FcGamma)嵌合分子的基本技术，用于患者定制的尘螨变态反应特异性免疫治疗。我们首次开发了一种新的、高重复性的鉴定IgE模拟表位多肽的方法，方法是引入一种使用螨类过敏患者的过敏原特异性免疫球蛋白进行预筛选的程序。我们还开发了利用动物细胞表达系统生产重组IgE模拟表位-FcGamma嵌合体的基本技术策略。

House dust mite allergy is a serious social problem, and establishment of its curable therapy is an urgent issue also in our country. Objective of this study is to develop basic technologies for the generation of sensitized allergen-specific IgE mimotope-human IgG antibody constant region (Fcgamma) chimeric molecules for patient-tailored specific immunotherapy against dust mite allergy. We first developed a new and highly-reproducible method for identification of the IgE mimotope peptides by introducing a pre-screening procedure using allergen-specific IgG from mite-allergic patients. We also developed basic technical strategies for production of recombinant IgE mimotope-Fcgamma chimeras using animal cell expression systems.