Analysis of gene expression of Helicobacter pylori VacA

为了确定幽门螺杆菌产生VacA毒素的刺激因素，我们在控制某些添加剂浓度的情况下对培养条件进行了考察。在含有5%小牛血清+0.1%环糊精的液体培养基中，在37℃、含5%CO2的环境空气中培养72 h，细菌生长和毒素产量最高。我们还利用抗VacA抗体结合柱层析法研究了细菌胞浆蛋白对VacA基因表达和分泌的影响，重点研究了成熟毒素和成熟毒素之间的相互作用。

To define the stimulatory factor of VacA toxin production from Helicobacter pylori, we have examined the culture condition under controlling the concentration of some additions.The liquid culture medium containing 5% bovine serum plus 0.1% cyclodextrin conferred highest bacterial growth and toxin production at 37oC for 72 h-cultivation in ambient air containing 5% CO2. We have also assessed whether bacterial cytosolic protein contributes gene expression and secretion of VacA focusing on interaction of mature and in mature toxin using anti-VacA antibody conjugated column chromatography.