Challenging study to clarify mechanisms of multiple organ failure

ATP参与细胞内和细胞外信号传导。中性粒细胞（PMNs）在炎症反应中起着关键作用。脓毒症患者血浆ATP和CD11b水平明显高于健康对照组，且CD11b与血浆ATP相关。这些数据表明，受损细胞或激活的炎症细胞释放ATP通过ATP受体刺激PMNs，引发败血症的炎症。脓毒症引起的DIC与高死亡率相关。评价脓毒症致DIC患者PMN变化的功能和可变形性。我们明确了PMN的变形能力和激活与脓毒症诱导的DIC的严重程度相关。利用化学传感器（PMAP-1, MitoAP-1）建立了人体PMNs中ATP的荧光成像。该方法将有助于了解ATP在PMNs中的动态，并阐明其在炎症中的多种生理功能。

ATP is involved in both intra and extracellular signaling. Neutrophils (PMNs) have a pivotal role in inflammation response. In sepsis patients, plasma ATP levels and CD11b were significantly higher than healthy controls, and the CD11b correlates with plasma ATP. These data suggest that ATP release from damaged cells or activated inflammatory cells stimulates PMNs via ATP receptors and triggers inflammation in sepsis. Sepsis-induced DIC is associated with a high mortality rate. The function and deformability of PMN change in patients with sepsis induced DIC was evaluated. We clarified that PMN deformability and activation correlated with the severity of sepsis-induced DIC. Fluorescence imaging of ATP in human PMNs with chemosensors (PMAP-1, MitoAP-1) was established. This method would contribute to understanding the dynamics of ATP in PMNs and elucidating their diverse physiological functions in inflammation.