Coordination Funds

尽管果蝇在实验室中作为农业害虫和遗传驮马具有重要意义，但它们对环境温度变化的反应和适应尚不清楚。了解对温度的反应不仅具有基本的生物学意义，而且对研究人员如何处理和储存这种重要的模式生物具有重要意义。此外，由于气候变化改变温度的速度在很大程度上阻碍了基因组的适应，并扩大了像铃木果蝇这样的害虫的地理范围，像果蝇这样的冷血动物如何改变它们的生理和行为以在广泛的温度范围内茁壮成长的问题将与控制努力密切相关。这个拟议的研究单位将采取协同的、多学科的方法，在实验室和实地研究黑腹果蝇和铃木果蝇的这个问题。我们将使用最先进的显微镜和定量图像分析来研究温度如何影响生长和形态发生的稳健性和速度。我们将把脂质组学的前沿方法与饮食操作相结合，进一步阐明饮食脂质对果蝇热行为的贡献。我们将量化染色质动力学和线粒体变异的作用-两者都显示出惊人的温度敏感性-并研究胰岛素信号介导的代谢变化的作用。最后，我们将揭示野外微生物组的季节变化，并测量它如何影响信号，脂质组成和活温范围。通过融合这些不同的观点，我们的研究结果将揭示这些相互作用因素在温度适应这一重要问题中的综合作用

Despite the importance of Drosophilids as agricultural pests and genetic workhorses in the lab, their response and acclimatization to environmental temperature variations are not well understood. Understanding the response to temperature is not only of basic biological interest, but also has important implications for how researchers work with and store this important model organism. Furthermore, as climate change alters temperature at a pace that largely prevents genomic adaptations, and expands geographical ranges of pests like Drosophila suzukii, the question how cold-­blooded animals like Drosophila alter their physiology and behavior to thrive over a wide range of temperatures will be of strong relevance to control efforts.This proposed Research Unit will take a synergistic, multidisciplinary approach to studying this problem in Drosophila melanogaster and Drosophila suzukii, both in the laboratory and in the field. We will use state of the art microscopy and quantitative image analysis to investigate the how temperature influences the robustness and speed of growth and morphogenesis. We will combine cutting edge approaches in lipidomics with dietary manipulations to further elucidate the contribution of dietary lipids to Drosophila thermal behavior. We will quantify the role of chromatin dynamics as well as mitochondrial variation – both known to display striking temperature sensitivity – and investigate the role of metabolic changes mediated by insulin signaling. Finally, we will uncover seasonal variation of the microbiome in the field, and measure how it influences signaling, lipid composition and the viable temperature range. By fusing these different perspectives, our findings will reveal the combined roles of these interacting factors in the important problem of the acclimatization to temperature