Development of a new pretreatment method for plasma endotoxin assay

1.我们设计了一种新的预处理方法（新的PCA方法），允许测量总内毒素。通过内毒素特异性分析测定人血浆中的含量。该方法可用于革兰阴性菌败血症的早期快速诊断.用此方法结合内毒素特异性鲎试验，可检测内毒素与脓毒症、感染性休克、胃肠道穿孔、烧伤、DIC（弥散性血管内凝血）等症状的关系。和多器官衰竭阐明了3.发现内毒素和多种细胞因子与上述症状的发生有关.许多研究人员，临床医生。日本和外国的临床检查人员要求我们使用这种方法，所以我们正在使用这种方法。该方法是诊断深部真菌病的上级方法.因此，我们相信这种方法将有助于拯救人类生命。

1. A new pretreatment method(the new PCA method), which we devised, permitted a measurement of the total endotoxin. content in human plasma by an endotoxin-spec'tic assay. This method was found to be useful for the early and rapid diagnosis of gram-negative bacterial sepsis.2. Using this method, combined with an endotoxin-specific limulus test, relation of endotoxin with symptoms such as sepsis, septic shock, gastrointestinal tract perforation, burns, DIC(disseminated intravascular coagulation). and multiple organ failure. was elucidated.3. It was found that endotoxin and many cytokines were concerned as triggers for the initiation of these symptoms mentioned above.4. Many researchers, clinicians. and clinical inspectors of Japan and, foreign countries are asking us to use this method, so we are making this method available.5. This method was applied as the superior method for diagnosis of deep-seated mycosis.6. Consequently, we believe this method will contribute to saving human lives.