Dualer, gepulster optisch-parametrischer Oszillatorlaser

申请资金用于定制双光学参量振荡器激光系统，用于使用泵浦-探测激发的光声成像和光谱学。与传统的OPO激光器相比，该系统将在可见光和近红外波长范围内提供独立控制的波长可调激发，脉冲之间的可变时间延迟（从1 ns到1000 ns），快速（脉冲到脉冲）波长调谐，高脉冲能量（> 70 mJ）和高重复率（200 Hz）。这不仅能够开发、全面评估和进一步完善用于体外PA成像的新型造影剂和基因表达的报告蛋白，而且还将为该技术在临床前和长期临床研究中的体内应用提供基础。双OPO激光器将克服我们小组目前可用的OPO激光系统所带来的限制。

Funding is requested for a custom-made dual optical parametric oscillator (OPO) laser system for photoacoustic (PA) imaging and spectroscopy using pump-probe excitation. In contrast to conventional OPO lasers, this system will provide independently controlled, wavelength-tuneable excitation across the visible and near-infrared wavelength region, variable time delays (from 1 ns to 1000 ns) between the pulses, fast (pulse-to-pulse) wavelength tuning, high pulse energies (> 70 mJ) and high repetition rates (200 Hz). This will not only enable the development, comprehensive evaluation and further refinement of novel contrast agents and genetically expressed reporter proteins for PA imaging in vitro but will also provide the basis for the translation of this technology to in vivo applications in preclinical and, in the longer term, clinical studies. A dual-OPO laser will overcome the limitations imposed by the OPO laser systems currently available to our group.