Analysis of Interactions in High Molecular Weight Protein Complexes by Using Segment Label and Cutting-edge NMR Technologies

为了开发具有生物重要性的高分子量蛋白质复合体结构分析的新方法，对链段标记和转移交叉饱和(TCS)和剩余偶极耦合(RDC)等新的核磁共振技术的应用进行了研究。利用天花粉蛋白作为呼吸链的最终电子转移络合物，即细胞色素c与细胞色素c氧化酶的络合物，我们成功地测定了细胞色素c与细胞色素c氧化酶直接作用的氨基酸残基。

To develop new methodologies for structural analysis of biologically important high-molecular-weight protein complexes, applications of the segment label and new NMR techniques such as the transfer cross-saturation (TCS) and residual dipole coupling (RDC) were examined. By using TCS for the final electron transfer complex in the respiratory chain, the complex between cytochrome c and cytochrome c oxidase, we successfully determined the amino acid residues of cytochrome c directly interacting with cytochrome c oxidase.