Construction of new breeding methods used the control of matylating pattern

DNA甲基化是基因表达调控的重要机制之一。这种模式会随着环境的变化而改变，比如压力和食物，改变后的模式会传递给下一代。因此，控制甲基化模式有望成为一种不伴随DNA序列改变的育种新方法。在这个项目中，甲基化模式与孔雀鱼的生长、耐热性和行为等几个特征之间的相关性进行了研究。在生长激素基因中，比较了5‘上游区域的甲基化模式，但没有观察到明显的甲基化模式与身体大小的相关性。在耐热性和行为的情况下，没有观察到相关性。另一方面，非致死热处理导致了致死温度下的耐热性。这是一种很有前途的培育耐热个体的新方法。构建了测量行为的方法。

DNA methylation is one of the most important mechanisms for the control of gene expression. This pattern is changed by environment, such as stress and food, and changed pattern is transferred to next generation. Therefore, the control of methylation pattern is expected as new breeding method which is not accompanied by change of DNA sequence. In this project, the correlation between methylation patterns and several traits, such as growth, thermal tolerance and behavior, were examined in the guppy. In the case of Growth hormone gene, the methylation pattern of 5' upstream region were compared, however, clear correlation was not observed between methylation pattern and body size. In the case of thermal tolerance and behavior, correlations were not observed. On the other hand, non-lethal heat treatment leads thermal tolerance at lethal temperature was decided. It is promising as the new method to produce thermal tolerant individual. The method to measure the behavior was constructed.