NMR Investigation of the Regulation of Gene Expression by RNA Thermometers

核糖核酸寡核苷酸（rna）参与基因表达的调控。RNA对各种外部刺激作出反应，如小代谢物（核糖开关RNA）浓度的变化，以及温度的变化（RNA温度计）。外部刺激转化为二级和三级结构的变化，通常是在变构部位。因此，基因表达可以在转录或翻译水平上进行调节。在本提案中，我们将使用静态和时间分辨核磁共振光谱来表征在纳伯豪斯小组中发现的新型RNA温度计的结构动力学（未发表的结果）。在第一个资助期，我们开发了一种方法，使我们能够根据热力学测量来描述单个碱基对的稳定性。此外，我们还开发了一种核磁共振温度跳变探头。在第二个资助期，我们将扩展我们对新型RNA温度计的研究，并将应用时间分辨核磁共振测量来确定RNA温度计开放信号状态的动力学和寿命。

Ribonucleic acid oligonucleotides (RNAs) are involved in the regulation of gene expression. RNAs respond to various external stimuli such as changes in the concentration of small metabolites (riboswitch RNAs) but also changes in temperature (RNA thermometers). The external stimulus is transformed into a change in secondary and tertiary structure, often at allosteric sites. Thereby, gene expression can be regulated both at the level of transcription or translation. In this proposal, we will characterize the structural dynamics of novel RNA thermometers discovered in the Narberhaus group (unpublished results) using static and time-resolved NMR spectroscopy. In the first funding period, we have developed a method that allows us to delineate the stability of individual base pairs based on thermodynamic measurements. In addition, we have developed a NMR temperature jump probe. In the second funding period, we will extend our studies to novel RNA thermometers and will apply time-resolved NMR measurements to determine the kinetics and life times of open signalling states of RNA thermometers.