Nanoseond Ultra-Rapid Freeze Quenching for Time-resolved Structural Biology

用于时间分辨结构生物学的纳秒超快速冷冻淬火

• 批准号: 451906961
• 负责人: Professor Dr. Harald Schwalbe
• 金额: --
• 依托单位: Institut für Organische Chemie und Chemische Biologie
• 依托单位国家: 德国
• 项目类别: New Instrumentation for Research
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/451906961?language=en
• 关键词: Nanoseond; Ultra; Rapid; Freeze; Quenching

The application aims to develop instrumentation for the time-resolved studies of biochemical processes, yielding 3D molecular movies recorded with atomic resolution in real time. Application targets regular and custom-made equipment for pre-steady-state triggering, sampling, probing and detection, including optical, cryo-sedimentation and ultra-high vacuum systems, UHPLC sample delivery and the computation cluster upgrade. Such instrumentation will be essential for time-resolved structural biology, in particular solid state NMR and cryo-EM.

该应用程序旨在开发用于生物化学过程的时间分辨率研究的仪器，产生以原子分辨率实时记录的3D分子电影。应用目标是用于预稳态触发、采样、探测和检测的常规和定制设备，包括光学、冷冻沉降和超高真空系统、UHPLC样品输送和计算集群升级。这种仪器对于时间分辨结构生物学，特别是固态核磁共振和低温电子显微镜来说是必不可少的。