Study of liposomes as drug-carrier.

1.脂质体包裹的链霉素（L-SM）对小鼠沙门氏菌病非常有效，尽管游离SM无效。脂质体包封可明显降低SM的急性毒性。静脉注射L-SM后，SM在肝脏和脾脏中大量蓄积，并能有效抑制Saltenin在这些器官中的生长.阿霉素（Adriamycin，Ad）包载于抗人甲<alpha>胎蛋白（AFP）单克隆抗体脂质体中，能选择性地与产生AFP的人肝癌细胞结合，抑制其体外生长，并能抑制裸鼠皮下或肝包膜下人肝癌的生长。脂质体中的包封降低了Ad.3的毒性。灌胃脂质体包封的胰岛素仅在四只链脲佐菌素诱导的糖尿病小鼠中的一只中降低血糖，并且观察到血糖降低与胰岛素的血液浓度之间没有关系。腹膜内注射脂质体包埋的胰岛素降低了6只糖尿病小鼠中的4只的血糖，并且在这种情况下，血糖的降低与胰岛素的血液浓度一致。这些结果表明，改进脂质体的制备是必要的，以实现成功的口服胰岛素治疗糖尿病。

1. Liposome-entrapped streptomycin(L-SM) was very effective against salmonellosis in mice, although free SM was ineffective. Entrapment in liposomes markedly reduced the acute toxicity of SM. Large amounts of SM accumulated in the liver and spleen after i.v. injection of L-SM, and growth of salmonella in these organs was effectively inhibited.2. Adriamycin(Ad) entrapped in anti-human <alpha> -fetoprotein(AFP) monoclonal antibody-conjugated liposomes bound selectively to AFP-producing human hepatoma cells and inhibited their growth in vitro, and also inhibited the growth of the human hepatoma implanted under the skin or the capsule of the liver of nude mice as assessed from the tumor-weight and blood concentraion of AFP. Entrappment in liposomes reduced the toxicity of Ad.3. Intragastric administration of liposome-entrapped insulin reduced blood sugar in only one out of four streptozotocin-induced diabetic mice, and no relation was observed between the decrease of blood sugar and blood concentration of insulin. Intraperitoneal injection of liposome-entrapped insulin reduced blood sugar in four out of six diabetic mice, and in this case the decrease of blood sugar coincided with blood concentration of insulin. These results indicate that improvement in the preparation of liposomes should be necessary to achieve successfull oral administration of insulin for therapy of diabetes mellitus.