Role of camalexin and sulfur nutrition in microbial assembly

在SPP解密的第一个资助期内，对根际微生物群落的组成及其动态的认识取得了很大进展。有明确的证据表明，植物形成微生物群落结构，很可能是通过根分泌物，也表明细菌已经发展出各种适应能力，以便在根际生态位中茁壮成长。我们通过鉴定Camalexin作为影响拟南芥植物与单个细菌菌株相互作用的代谢物之一以及微生物组功能，为这一知识做出了贡献。我们已经确定了一个影响Camalexin合成和渗出以及与细菌共培养结果的基因。我们还表明，这些结果取决于硫的可获得性。在这个项目中，我们将确定Camalexin如何影响微生物的功能，以及芽和根如何沟通以控制其合成和分泌。利用植物和微生物硫酸盐同化的操作，我们将确定硫营养是否以及如何有助于微生物组组装的控制。

During the first funding period of the SPP DECRyPT a great progress has been made in the knowledge of composition of rhizospheric microbiomes and their dynamics. There is clear evidence that plants shape microbiome structures, most probably by root exudates, and also that bacteria have developed various adaptations to thrive in the rhizospheric niche. We have contributed to this knowledge by identification of camalexin as one of the metabolites affecting the interaction of Arabidopsis plants with individual bacterial strains as well as the microbiome function. We have identified a gene affecting camalexin synthesis and exudation and the outcome of cocultivations with bacteria. We also showed that these outcomes are dependent on sulfur availability. In this project we will determine how camalexin affects microbial function and how shoots and roots communicate to control its synthesis and exudation. Using manipulations of plant and microbial sulfate assimilation we will establish whether and how sulfur nutrition contributes to control of microbiome assembly.