Genetic code expansion tools to introduce post-translational modifications (C02)

蛋白质翻译后修饰(PTM)对于调节细胞生理的各个方面具有重要意义。在这个CRC中，我们希望利用遗传密码扩展方法--结合体内化学和酶反应--在体内特异性地将PTM如琥珀酸化和泛素化整合到蛋白质中。这些方法将帮助我们破译它们在核小体动态、DNA复制、转录和蛋白质定位方面的作用--与该中心的其他小组合作，并在慕尼黑地区。此外，这些工具将受益于普遍适用于各种生物学问题的优势。

Protein post-translational modifications (PTMs) are important for regulating diverse aspects of cellular physiology. Within this CRC we want to leverage genetic code expansion approaches - in conjunction with in vivo chemistries and enzymatic reactions – to site-specifically incorporate PTMs such as succinylation and ubiquitination into proteins in vivo. Such approaches will help us deciphering their roles – in collaboration with other groups of this CRC and in the Munich area – in nucleosome dynamics, DNA replication and transcription and protein localization. Furthermore these tools will benefit from the advantage of being generally applicable to diverse biological questions.