Development of the method for continuous and in situ determination of ions in circulating blood.

研制了一种新的血钠钾浓度连续测量系统。一个小的体外循环，血液通过钠和钾敏感的玻璃电极。将该系统应用于有意识的大鼠和狗，证明了该方法在生理分析中的适用性。[Na]和[K]的测量使用Na和K敏感玻璃电极和安装在电解质分析仪（SERA-230，堀场，京都）温度控制单元上的参比电极。在本系统中，用3.3 mol/1 KCl琼脂桥取代了商用分析仪中使用的液体结，调节琼脂的pH和Na浓度与血液的pH和Na浓度。在生理范围内，测定Na浓度的准确度约为1.5 meq/1， K浓度的准确度小于0.1 meq/1。红细胞压积42% ~ 51%对读数的影响在0.1 mV以内。动物实验在大鼠和狗身上进行，并用于分析饮酒行为。结果表明，该系统具有足够的灵敏度，可以检测饮水行为引起的Na浓度变化，也可以检测饮用水的盐度差异。这些结果表明，该方法不仅是生理学研究的潜在工具，而且在肾脏学和心血管外科领域的临床应用也是一个潜在的工具。

A new continuous measuring system for blood Na and K concentrations was developed. A small extracorporeal circulation was made and the blood was passed through Na and K sensitive glass electrodes. Applying the system to conscious rats and dogs, applicability of the method for physiological analyses was shown.Measurements of [Na] and [K] were made using Na and K sensitive glass electrodes and a reference electrode mounted on a temperature control unit of an electrolyte analyzer (SERA-230, Horiba, Kyoto). In the present system, the liquid junction used in the commercial analyzer was replaced by 3.3 mol/1 KCl agar bridge adjusting pH and Na concentration of the agar to those of blood. The accuracy of the determination was about 1.5 meq/1 of Na and less than 0.1 meq/1 of K concentration within physiological range. The effect of erythrocytes with 42 to 51% of hematocrit on reading was within 0.1 mV.Animal experiments were performed on rats and dogs, and used for the analysis of drinking behavior. The results showed that the system is sensitive enough to detect the changes in Na concentration consequent to drinking behavior and also to detect difference of salinity of drinking water. These results indicate that the present method is a potential tool for not only for physiological studies but also for clinical use in the fields of nephrology and cardio-vascular surgery.