Intramyocardial magnetic targeting of cardiac myocytes

干细胞来源的心肌细胞移植是一种很有前途的治疗方法。目前的数据表明，移植细胞的少量植入和存活表明需要提高移植效率以及移植细胞的短期和长期植入。为了解决移植效率低下的问题，我们假设有可能在小鼠心脏细胞替代疗法模型中大幅增加移植心肌细胞的植入。在靶组织中保留的移植细胞的精确和可靠的定量是必须的，并将通过一种完善的基于实时pcr的方法进行。将通过移植心肌细胞荧光素酶成像进行纵向分析。

Transplantation of stem cell derived cardiac myocytes is a promising therapeutic approach. Present data demonstrate a minor engraftment and survival of transplanted cells pointing to a need to improve the transplantation efficiency as well as the short- and long-term engraftment of transplanted cells. In order to address the issue of poor transplantation efficiency we postulate strategies that have the potential to substantially increase the engraftment of transplanted cardiac myocytes in a murine model of cardiac cell replacement therapy. A precise and reliable quantification of transplanted cells retained in the target tissue is mandatory and will be performed by a well-established real-time-PCR based approach. Longitudinal analysis will be performed by luciferase imaging of transplanted cardiomyocytes.