Treatment of Periodontitis using stem cell like epithelial cell rests of Malassez

马拉色氏上皮细胞(ERM)是一种容易获得干细胞样细胞的来源。我们以前的工作已经成功地证明了一种ERM突变体(ERM的分离克隆)可以通过抑制牙齿和牙槽骨之间的强直来维持健康的牙周空间。本研究旨在利用申请人先前分离的克隆模型来修复丢失或损伤的牙周膜(PDL)组织，以恢复牙齿的形态和功能。阶段1：我们从猪的PDL组织和ERM的分离克隆中获得了原代ERM。我们分离了克隆人1-10。通过ERM细胞标志物的免疫荧光染色进行表征。

Epithelial cell rests of Malassez (ERM) are an easy source of stem cell-like cells. Our previous work has successfully demonstrated that an ERM variant (isolated clone of ERM) can maintain healthy periodontal space by inhibiting ankylosis between the tooth and alveolar bone.This study aims to use the applicant's previously isolated clone model to repair the lost or injured periodontal ligament (PDL) tissue to restore tooth form and function.Phase-1: We obtained primary ERM from PDL tissues of porcine and isolated clones of ERM. We isolated clones 1-10. The characterization was done using immunofluorescence staining of ERM cell markers.