Regulation of matrix metalloprotease activity during proteolytic invasion of human macrophages (B13*)

该项目研究了基质金属蛋白酶（MMPs）在巨噬细胞蛋白水解侵袭中的作用。在细胞内，MMP沿沿着微管双向移动，受特定驱动蛋白和动力蛋白马达蛋白以及RabGT家族成员的调节。发现了一种新的MMP依赖性细胞外基质蛋白水解的驱动蛋白调节剂。将研究这种驱动蛋白在MT 1-MMP循环途径中的作用。开发抑制肽并测试其在病理生理学相关背景下对巨噬细胞侵袭的调节。

The project studies the role of matrix metalloproteases (MMPs) in the proteolytic invasion of macrophages. Within cells, MMPs travel bidirectionally along microtubules, regulated by specific kinesin and dynein motor proteins and members of the RabGTPase family. A novel kinesin regulator of MMP-dependent extracellular matrix proteolysis was discovered. The role of this kinesin in the recycling pathways of MT1-MMP will be studied. Inhibitory peptides are developed and tested for modulation of macrophage invasion in a pathophysiologically relevant context.