Mass-Marking Method for Fish Eggs and Larvae

该项目计划建立一种大量标记鱼卵和仔鱼的方法。在香鱼（Plecoglossus altivelis）的前期研究基础上，建立了香鱼卵和仔稚鱼浸泡法标记耳石的方法。(1)茜素络合酮（ALC）、四环素（TC）和钙蛋白酶（CAL）分别呈现明显的红红色、黄色和淡黄色荧光环。ALC和TC处理的最佳条件分别为50-200 mg/l和200-300 mg/l，处理时间为1天左右。CAL不太成功。(2)标记保持和标记效果TC和ALC处理对5个月至2年的香鱼和真鲷的存活和生长没有不利影响，并且每个处理的鱼都有清晰的荧光标记。(3)耳石标记的可用性这一技术已被证实可用于约25个物种，包括海洋和淡水物种。1987年和1988年在大分县的一个小海湾进行了ALC处理的真鲷的标记重捕试验。实验是成功的，我们证实了这种方法在实地调查的可用性。

This project was planned to establish a method of mass-marking fish eggs and larvae. Based on thd preliminary works on the ayu, Plecoglossus altivelis, otolith-tagging by immersion eggs and larvae in solution of fluorescent sudstances was nesly developed as follows.(1) Screening of marking agents and optimum treatment condition Alizarin complexone (ALC), tetracycline (TC) and calsein (CAL) presented a distinct scarlet-pink, yellow and yellowish hreen fluorescent ring, respectively. Optimum conditions for ALC and TC teatment was 50-200 mg/l and 200-300 mg/l for about 1 day. CAL was less successful.(2) Mark retention and effect of marking treatment TC and ALC treatment had no unfavourable effect on survival and growth of the ayu and the red sea bream Pagrus major reared for 5 months to 2 years after treatment, and clear fluorescent marks were confirmed in every fish treated.(3) Availability of otolith-tagging This technique was confirmed to be available for about 25 species including both marine and freshwater species. Mark-recapture experiment of ALC-treated red sea bream was conducted in a small cove Oita prefecture in 1987 and 1988. Experinents were successful and we confirmed the availability of this method in a field survey.