Isotope mass spectrometer (GC MS-IRMS-ASE)

土壤学、有机地球化学和食品技术工作组联合申请气相色谱-质谱仪-同位素质谱仪-加速溶剂萃取-耦合。该仪器将用于以下科学领域：㈠确定植物材料、土壤和沉积物中草药和微生物代谢物的数量，并确定所测代谢物的同位素比率;（ii）结合联合收割机，（13 C，15 N，D，18 O）与微生物生物标志物的组分特异性同位素分析（PLFA，氨基糖），以分析和量化土壤和沉积物中单个微生物群体的特定代谢途径，以及量化特定微生境中的元素周转;（iii）该仪器将能够研究在食品特有储存条件下复杂系统中脂质和蛋白质的共氧化，并确定所形成的氧化产物的同位素比率;（四）最后，该仪器将有助于通过追踪它们的成岩转化产物来确定土壤和沉积物中最近的蓝藻输入。复杂介质（土壤、植物、沉积物、食品）中的代谢物研究是土壤、地质和生物科学之间的一个接口，需要采用跨学科方法，并需要基尔大学各个院系和研究所之间的合作。共享的分析平台是这种机构间合作的先决条件。所提出的同位素质谱仪是这种分析平台的核心部分。

The working groups soil science, organic geochemistry and food technology jointly apply for a Gas Chromatograph-Mass Spectrometer-Isotope Mass Spectrometer-Accelerated Solvent Extraction-Coupling. The instrument will be used in the following scientific fields: (i) for the determination and quantification of herbal and microbial metabolites in plant material, in soil and in sediments, as well as for the determination of isotope ratios of the measured metabolites; (ii) to combine position-specific stable isotope labelling of organic substances (13C, 15N, D, 18O) with component-specific isotope analysis of microbial biomarkers (PLFA, amino sugar), in order to analyze and quantify specific metabolic pathways of individual microbial groups in soils and in sediments, as well as to quantify element turnover in specific microhabitats; (iii) the instrument will enable to study the co-oxidation of lipids and proteins in complex systems under characteristic storage conditions for food and the determination of isotope ratios of the formed oxidation products; (iv) and finally the instrument will contribute to the determination of the recent cyanobacterial input in soil and in sediments by tracing their diagenetic conversion products.Component specific stable isotope analysis of metabolites in complex media (soils, plants, sediments, food) represents an interface between soil-, geo- and biosciences and requires an interdisciplinary approach and cooperation between the individual faculties and institutes of the Christian-Albrechts-University Kiel. A shared analytics platform is a prerequisite for such an interinstitutional cooperation. The proposed isotope mass spectrometer is a central part of such an analytics platform.