Multiphoton laser scanning microscope for intravital imaging applications

这项提议旨在建立一个多光子成像装置，用于生命内成像应用。该装置将设在动物收容所封闭的动物手术室内，这将使用户能够进行纵向重复检查，要求在不同的时间点将动物带进和带出收容所。申请者将重点放在肿瘤学、心血管和免疫学的应用上，明确重点是研究和跟踪先天免疫细胞和获得性免疫细胞。因此，将对心血管系统、心肌、初级和次级淋巴器官进行成像。申请的设置是为这些应用量身定做的，因为它允许跟踪组织中的细胞和快速生命内成像。该显微镜将嵌入现有成像核心设施的结构中，并将提供高水平活体成像的可能性，并将填补现有成像平台中未得到满足的需求。

This proposal aims to establish a Multiphoton imaging unit for intravital Imaging applications. The set-up will be situated within the animal operation theatre enclosed in the animal housing facility, which will enable the users to perform longitudinal repetitive investigations that require the animals to be taken in and out of the housing facility at various time points. The applicants are focusing on oncological, cardiovascular and immunological applications, with a clear focus on the investigation and tracking of innate and adaptive immune cells. Therefore imaging of the cardiovascular system, myocardium, primary and secondary lymphoid organs will be performed. The set-up applied for is tailored for these applications in that it allows tracking of cells in tissue and fast intravital imaging. The microscope will be embedded within the structure of the existing imaging core-facilities and will provide the possibility for high-level intravital imaging and will fill an unmet need within the available imaging platforms.