Modulation of the in situ pellicle by alkanna tinctoria roots in oleum oliva (olive oil)

前言：龋齿和腐蚀性的发生是基于牙齿表面的生物黏附过程。因此，表膜作为这些生理和病理生理过程和牙齿表面的媒介。可以通过应用生物或仿生制剂来有意地修饰膜层，以提高保护性能，例如防止侵蚀的保护效果。此外，还可以减少微生物的粘附性。目的：这项拟议的临床-实验研究旨在研究橄榄油中的脂肪胶束作为一种可能的生物转运系统来运输红豆杉根中的活性物质。此外，还将在原位条件下评估橄榄油中的丹参根对口腔初始生物黏附的影响。因此，主要的焦点将放在橄榄油中丹参根作为防腐剂的适用性、膜超微结构的改变以及可能减少初始细菌定植的可能性上。方法：将牛牙釉质片口腔携带(n=6)。在膜形成1 分钟后，受试者用10毫升的测试液(对照不漂洗，碱茅根在橄榄油中，碱茅根不含橄榄油)冲洗10分钟。然后，对每个测试液和每个受试者进行以下实验：淀粉酶、溶菌酶、过氧化物酶和GTF的酶活性测定。此外，还将进行荧光显微镜观察细菌黏附和钙/磷释放的光度测量。此外，还将对4名受试者用透射电子显微镜观察膜层超微结构中的脂质胶束和与盐酸孵育后的膜层超微结构。此外，脂质胶束和丹参有效物质的结合可以作为牙科预防中创新生物学方法发展的基础。

Introduction: The incidence of caries and erosion is based on bioadhesion processes at the tooth surface. Thereby, the pellicle serves as a mediator of these physiological and pathophysiological processes and the tooth surface. It is possible to modify intentionally the pellicle layer by the application of biological or biomimetic preparations in order to improve the protective properties, like the protective effect against erosion. Further, the adhesion of microorganisms can be reduced. Aims: The proposed clinical-experimental study aims to examine the application of lipid micelles from olive oil as a possible biological transport system for bioactive substances from Alkanna tinctoria roots. Furthermore, the effect of Alkanna tinctoria roots in olive oil on the initial oral bioadhesion will be evaluated under in situ conditions. Thereby, a main focus will be the applicability of Alkanna tinctoria roots in olive oil as a protection against erosion, the modification of the pellicle ultrastructure and the possible reduction of initial bacterial colonization.Methods: Bovine enamel slabs will be carried intraorally (n= 6 subjects). After 1 min of pellicle formation, the subjects rinse with 10 ml of the test solutions (control without rinsing, Alkanna tinctoria roots in olive oil, Alkanna tinctoria roots without olive oil) for 10 min. Afterwards, the following experiments will be carried out per test solution and per subject: Enzyme activity measurements of amylase, lysozyme, peroxidase and GTF. Furthermore, fluorescence microscopy to visualize bacterial adhesion and photometrical measurements of calcium/phosphate release will be performed. In addition, a visualization of lipid micelles in the pellicle ultrastructure and the pellicle ultrastructure after incubation with HCL will take place in 4 subjects with transmission electron microscopy.Perspective: The investigations will contribute fundamentally to the understanding of the modification of oral bioadhesion processes. Furthermore, the combination of lipid micelles and the potent substances from Alkanna tinctoria roots could serve as a basis for the development of innovative biological approaches in preventive dentistry.