confocal STimulated Emission Depletion (STED) microscope system

在解剖研究所，目前只有配备了荧光技术的局部显微镜。随着解剖、生化、生理等学科的科学进步，对高分辨率的共聚焦显微镜提出了更高的要求。这样一项新技术就是sted刺激排放耗尽技术。它允许低至30纳米的成像分辨率，属于高分辨率技术，需求不断增加。它使申请人能够对许多亚细胞结构和蛋白质进行高质量的荧光成像，并允许解决许多细胞生物学问题。应用STED显微镜的激光配置可以同时成像多达八种不同的荧光色，包括其精确的激发，这允许多用途的应用。在基尔大学没有超分辨率的显微镜。与此同时，跨部门的高需求不仅存在于支持现有资助项目（如SFB877“蛋白水解作为病理生理学中的调节事件”，GRK2154“材料4脑”和各种项目资助），而且还存在于启动新的资助项目。

In the Anatomical Institute are currently localized microscopes equipped with epifluorescence technology only. For the scientific progress of groups from the anatomical, biochemical and physiological departments a high demand on confocal microscopes with high resolution technology exists. Such a new technology is the STED-Stimulated Emission Depletion. It permits imaging with a resolution of low as 30 nm, and belongs to the high resolution technology with increasing demand. It enables the applicants to perform fluorescence imaging in extremely high quality of many subcellular structures and proteins and permits to solve many cell biological questions. The laser configuration of the applied STED microscope enables the imaging of up to eight different fluorochromes at the same time including its exact excitation, which permits versatile applications. At the university of Kiel no super resolution microscope exists. At the same time, a high demand across departments exists not only to support existing funded projects (f. e. SFB877 “Proteolysis as a Regulatory Event in Pathophysiology”, GRK2154 “Materials 4 Brain“ and various project fundings) but also to initiate new funded projects.