Spectral Confocal Laser Scanning Microscopy

这里提出的光谱共焦激光扫描显微镜（CLSM）将取代我们的CLSM系统，这是在2002年购买。此外，它还将出售2012年购买的另一套系统。最近，由于探测器出现故障，我们不得不关闭旧仪器。因为，我们在学院的CLSM中有一个非常严重的瓶颈，许多研究人员在成像和测量所有样品方面遇到了巨大的问题（特别是细胞生物学和植物生物化学，发育生物学和神经生物学三个部门的主要用户）。现有的仪器非常灵活和灵敏，但目前的用户负荷非常高，甚至两个CRC和DFG研究生院的主要用户也无法对所有样品进行成像和测量。因此，作为替换购买，我们提出了一个CLSM系统，它可以补充现有系统，并允许上述部门的各个研究小组进行所有必要的实验。此外，在指导后，我们还希望允许其他研究小组的偶尔用户按需对他们的样品进行成像和测量。因此，需要高度灵活的CLSM系统，允许用户在不同波长下对固定样品和活体材料进行成像和测量。仪器还应允许FRET和FRAP测量。由于大量不同的样本和用户，重要的标准是快速成像速度，灵活性，高灵敏度，低光毒性和用户友好的系统。

The here proposed spectral confocal laser scanning microscope (CLSM) shall replace our CLSM system, which was purchased in 2002. Moreover, it shall disburden another system purchased in 2012. Recently, we had to shut down the old instrument after a defect of the detector. Since, we have a very severe bottleneck in CLSM at the faculty and a number of researchers have enormous problems to image and measure all their samples (especially the main users of the three departments Cell Biology and Plant Biochemistry, Developmental Biology and Neurobiology). The available instrument is very flexible and sensitive, but the user load is currently so high that not even the main users, which are involved in two CRCs and a DFG-graduate school, are able to image and measure all their samples. Therefore, as replacement purchase, we propose a CLSM system, which can complement the existing system and additionally allows the various research groups of the above-mentioned departments to perform all their necessary experiments. Moreover, after instruction, we would also like to allow occasional users of other research group to image and measure their samples on demand. Therefore, a highly flexible CLSM system is required, which allows users to image and measure fixed samples and living material at different wavelengths. The instrument shall also allow FRET and FRAP measurements. Due to the large number of different specimen and user, important criteria are fast imaging speed, flexibility, high sensitivity, low phototoxicity and a user-friendly system.