NEUROCHEMICAL STUDIES ON CNS DISTURBANCE CAUSED BY ORGANIC SOL VENTS

本文以神经元和胶质细胞标志蛋白为指标，通过动物实验研究了有机溶剂引起中枢神经系统损害的机制，以期为有机溶剂中毒的早期诊断和卫生标准制定提供基础资料。实验结果如下：（1）亚急性甲苯染毒组，大鼠分别暴露于300、1000、3000 ppm的甲苯中，每天8小时，每周6天，共2周。暴露后，测量神经元标记蛋白（γ-烯醇化酶）和神经胶质细胞标记蛋白（α-烯醇化酶、β-100蛋白、肌酸激酶-B）。α-烯醇化酶和α-烯醇化酶在小脑中显著增加，β-100蛋白在脑干中显著增加。(2)在慢性甲苯暴露中，大鼠暴露于100，300，1000 ppm，每天8小时，每周6天，共16周。小脑胶质细胞标志蛋白呈剂量依赖性增加。β-100蛋白在脑梗死组显著升高， ...更多信息 脑干和脊髓。(3)亚急性甲乙酮（MEK）染毒组，染毒剂量分别为200、630、2000 ppm，每天8小时，每周6天，共2周。将大鼠暴露于2000 ppm的正己烷，每天12小时，每周6天，持续24周。坐骨神经远端神经元和神经胶质细胞标志蛋白明显减少。但这些标记蛋白在CNS中没有显著变化。结果表明，这些指标可以很好地反映有机溶剂对中枢神经系统的影响，甲苯和MEK可能会导致中枢神经系统的胶质细胞增生。(5)大鼠腹腔注射甲苯80、250、800 mg/kg和甲基苯丙胺。用微透析法测定脑纹状体内多巴胺及其代谢产物DOPA和HVA的含量。观察大鼠行为学变化。苯丙胺组多巴胺及其代谢产物、行为学指标明显升高。甲苯800 mg/kg组行为明显增加，但多巴胺及其代谢产物无变化。结果表明，甲苯的作用机制可能与安非他明不同，甲苯可能会影响大脑的其他部分。少

The mechanism of the CNS disturbance caused by organic solvents are studied in the animal experiments using neuronal and glial cell marker proteins as indicators in order to gain some basic data for early diagnosis and hygienic standard of organic solvent poisoning. The summary of the results is as follows;(1) In the subacute toluene exposure, rats were exposed to toluene 300,1000,3000 ppm,8 hours a day, 6 days a week,for 2 weeks. After the exposure, neuronal marker protein (gamma-enolase) and glial cell marker proteins (alpha-enolase,beta-100 protein, creatinekinase-B) were measured. The alpha-enolase and alpha- enolase significantly increased in the cerebellum and beta-100 protein significantly increased in the brainstem.(2) In the chronic toluene exposure, rats were exposed to 100,300,1000 ppm, 8 hours a day, 6 days a week, for 16 weeks. The glial cell marker proteins dose-dependently significaqntly increased in the cerebellum. The beta-100 protein significaqntly increased in the br … More ainstem and spinal cord. (3) In the chronic n-hexane exposure, In the subacute methylethylketone(MEK) exposure,rats were exposed to MEK 200,630,2000 ppm, 8 hours a day, 6 days a week, for 2 weeks. rats were exposed to n-hexane 2000 ppm, 12 hours a day, 6 days a week, for 24 weeks. The neuronal and glial cell marker proteins significaqntly decreased in the distal sciatic nerve. But these marker proteins did not significantly change in the CNS. The resuls suggest that these markers could be good indicators of the effects of organic solvents on the CNS, and toluene and MEK might cause gliosis in the CNS. (5) Rats were injected intraperitoneally with toluene 80,250,800 mg/kg and metaamphetamine. Dopamine and its metabolites DOPA and HVA in the striatum of the brain were measured by using microdialysis method. The behavior of the rats were observed. The dopamine and its metabolites, and behavior significantly increased in amphetamine group. The behavior significantly increased but dopamine and its metabolites did not change in toluene 800 mg/kg group. The results suggest that the mechanism of the effect of toluene could be different from that of amphetamine, and toluene might affect other portions of the brain. Less