Histologic study of cellular response to graft material on hybrid-type vascular prosthesis.

小口径血管假体在长期通畅率方面仍然不令人满意。研究了一种混合血管假体，通过将活细胞与合成移植物相结合的方式来获得更好的通畅性。我们研究了内皮细胞在超细聚酯纤维移植物上的播种，并开发了过滤播种的方法，以利用大量的细胞。体外和体内研究表明，随着时间的推移，内皮细胞在移植物周围生长，并在植入后3个月保持内皮细胞的连续单层。然而，在吻合部位，内皮细胞单层在厚的内皮下组织上形成，并持续到宿主动脉的厚内膜。仅用ECs播种可能不足以防止嫁接失败。在接下来的研究中，内膜增生可能是实现小口径血管假体良好通畅的主要目标。

A small caliber vascular prosthesis remains unsatisfactory in regard with the long term patency rate. A hybrid vascular prosthesis has been investigated for achieving the better patency in the way of combining the living cells with a synthetic graft. We studied the endothelial cells (ECs) seeding to the ultrafine polyester fiber grafts and developed the filtration seeding method in order to utilize a large number of cells. The in vitro and in vivo study revealed that the ECs grew up around the grafts with time and maintain the continuous monolayer of the ECs three months after implantation. However, at the anastomotic sites, the ECs monolayer was formed on the thick subendothelial tissue which continued to the thick intima of the host artery. The ECs seeding only may not be enough to prevent the graft failure. The intimal hyperplasia may be the major target for achieving the better patency of the small caliber vascular prosthesis in the following research.