Molecular Construction on the Formation of Cell Wall by Immunofluorescence Microscopy

我们用免疫荧光显微镜观察了鼻甲七叶树长形上胚轴上皮层mt的排列。在暗生长和明暗生长条件下，mt的排列与伸长率有关，且在同一上胚轴上不同区域mt的排列不同。本研究结果表明，在伸长停止前，皮层的MTs的排列发生了从横向到斜向或纵向的变化。在两种生长条件下，MTs的取向似乎在薄壁细胞的外部比在内部更陡峭，并且在浅色-深色生长的上胚轴中改变得更早。采用快速冷冻和深度刻蚀技术对杨树悬浮培养细胞进行细胞壁多糖提取前后的细胞壁结构进行了观察。果胶降解酶处理后的细胞壁结构也进行了检测。我们的观察表明，化学和酶处理提取果胶后，纤维间的间隙或孔径增加。4%氢氧化钾处理后，纤维素微原纤维的有序间距发生了轻微变化，24%氢氧化钾处理后，纤维素微原纤维的有序间距发生了剧烈变化。这些结果表明，半纤维素，可能是木葡聚糖，可能在这种双子叶植物细胞壁中通过纤维间多糖键维持三维构象中起作用。

We examined the arrangement of cortical MTs in the elongating epicotyl of Aesculus turbinata by immunofluorescence microscopy using cryosections. Under both dark and light-dark growth conditions, the arrangement of MTs was associated with the rates of elongation and differed according to the region in the same epicotyl. The present results indicate that the change in the arrangement of cortical MTs from transverse to oblique or longitudinal ones occurs in the cortex before elongation stops. Orientation of MTs in both growth conditions seems to be steeper in the outer than in the inner parenchyma cells and to change earlier in the light-dark grown epicotyl.The architecture of the primary wall in poplar suspension-cultured cells was observed after rapid freezing and deep etching techniques before and after the sequential extraction of cell wall polysaccharides.The cell wall architecture was also examined after the treatment by pectin-degrading enzymes. Our observations showed that the inter-fibrillar spaces or pore size increases after extraction of pectins both with chemical and enzymatic treatments. The ordered spacing of cellulose microfibrils was only slightly altered after 4 % KOH-treatment and drastically altered after 24 % KOH-treatment. These results suggest that the hemicellulose, possibly xyloglucan, may have apossible role in maintaining the three-dimensional conformation by inter-fibrillar polysaccharide linkages in the cell wall of this dicotyledonous species.