New Development of Enzyme Utilization in Organic Solvents

在脂肪酶Saiken 100的催化作用下，伯、仲、叔醇在有机介质中与三甲基硅基烯有效地酰化，得到三甲基硅基乙酰酯。这种酶还能成功地使苯酚和大醇以及带有羧基的底物酰化。该酶还催化了三乙基硅酮和二甲基苯基硅酮的酰化反应。双烯酮具有高度应变的四元环和分子内烯醇-酯结构，是脂肪酶介导的有机溶剂中醇酰化的一种有效的酰化剂。与脂肪酶Amano PS的立体选择性极高，否定了在蛋白质表面催化的传统说法。剩余的醇和生成的酯可以通过简单的分馏分离。具有类似结构的β -内酯也进行了立体选择性开环反式酯化反应，是合成天然产物和生物可降解共聚物的重要原料。

By the catalytic action of a lipase, Saiken 100, primary, secondary, and tertiary alcohols were effectively acylated with trimethylsilylketene in organic media, to give trimethylsilylacetyl esters. The enzyme also successfully acylated a phenol and bulky alcohols as well as a substrate bearing a carboxyl group. The acylation with triethylsilylketene and dimethylphenylsilylketene was also catalyzed by the enzyme.Diketene having a highly strained 4-membered ring and intramolecular enol-ester structure was found to be a powerful acylating agent for lipase mediate acylation of alcohols in organic solvents. The stereoselectivity with a lipase, Amano PS,was extremely high which denied the conventional propose of the catalysis on the protein surface. The remaining alcohols and generated esters were able to be separated easily by simple fractional distilllation. beta-Lactones having the similar structure was also trans-esterified with ring opening stereoselectively which were very important starting materials for natural products synthesis and bio-degradable copolymers.