Immunoproliferative Microenvironment in the Spleen-An Immunohistological Study.

(本研究的目的)我们对大鼠脾中ED2阳性的巨噬细胞和抗体形成细胞形成的细胞花环进行了免疫组织学检测。本研究的目的是了解不同类型的免疫反应与玫瑰花环形成之间的关系，并考虑玫瑰花环作为免疫增殖微环境的功能作用。(结果)(1992)(1)我们证明了ED2阳性的巨噬细胞也在红髓中与红细胞形成玫瑰花环(发表于获得这项资助之前)。(2)我们检测了抗FITC抗体形成细胞的亚型，发现它们主要具有IgM亚型(未发表的数据)。(3)免疫电子显微镜分析正在进行。(1993)(1)我们制备了FITC-血蓝蛋白抗原，发现针对该抗原的抗体形成细胞也与ED2阳性的巨噬细胞在外PAL形成玫瑰花环(未发表资料)。我们还研究了感染疟原虫的大鼠脾。我们发现形成…的抗体在PALS中，更多的细胞和ED2阳性的巨噬细胞数量显著增加，并一起形成玫瑰花环(在一次国际会议上提出，准备)。(2)本项目站在一个尚处于初级阶段的研究领域--“免疫组织学”，有待于进一步研究。然而，我们提出这种方法是一种新的和非常重要的方法。为了展示这一领域的一些作物，我们制作并研究了一种大鼠实验性胰腺炎模型。我们发现炎症组织中巨噬细胞大量增多(胃肠病学，1993)，并用双重免疫染色技术(Arch.Organol.Cytol，1993)证实了巨噬细胞亚群的增殖(讨论和未来计划)。我们认为，ED2阳性巨噬细胞和抗体形成细胞的花环形成是一种普遍现象，不仅为抗体形成细胞对各种人工抗原的反应创造了微环境，也为抗体形成细胞对感染性微生物的反应创造了微环境。此外，它们还可能构成造血细胞谱系的微环境。我们计划用双色荧光探针对玫瑰花环进行免疫染色，并借助共聚焦激光扫描显微镜检查它们的三维结构。较少

(Aim of this study) We examined immunohistologically the cellular rosettes formed by ED2-positive macrophages and antibody forming cells in the rat spleen. The aim of this study is to know the correlation between various types of immune response and the rosette formation and to consider the functional role of the rosette as an immunoproliferative microenvironment.(Results)(1992)(1) We demonstrated that ED2-positive macrophages also form rosettes with erythroblasts in the red pulp cord (Published just before getting this grant). (2) We examined isotypes of anti-FITC antibody forming cells and found that they bore mainly an IgM isotype (unpublished data). (3) The immunoelectron microscopical analysis is now undergoing.(1993)(1) We prepared an antigen, FITC-hemocyanin, and found that antibody forming cells to this antigen also formed rosettes with ED2-positive macrophages in the outer PALS (unpublished data). We also studied Plasmodium-infected rat spleens. We found that antibody forming … More cells and ED2-positive macrophages considerably increased in number and formed rosettes together in the PALS (presented at an international congress, in preparation). (2) This project stands on a still preliminary field of research, "Immunohistology", which needs further study to be established. However, we propose this kind of approach as a new and very important one. In order to demonstrate some crops of this field, we have produced and studied a rat experimental pancreatitis model. We found a massive increase of macrophages in the inflamed tissues (Gastroenterology, 1993) and demonstrated a proliferation of macrophage subpopulations by the double immunostaining technique (Arch.Histol.Cytol., 1993).(DISCUSSION AND FUTURE PROJECT) We consider that the rosette formation of ED2-positive macrophages and antibody forming cells is a common phenomenon producing the microenvironment for the antibody forming cell response not only to various types of artificial antigen but also to infectious microorganisms. Furthermore, they may slso constitute the microenvironment for hemopoietic cell lineages. We plan to immunostain the rosettes with double color fluorescent probes and examine the three dimensional structure of them with the aid of a confocal laser scanning microscope. Less