Immunological Investigation on the adverse reaction of iodinated contrast media

我们在1993-1994年间的研究表明，对小鼠的IgE抗体产生可能有佐剂作用。此外，佐剂作用可能与白介素4的诱导免疫反应有关(1995年6月在芬兰图尔库举行的造影剂研究会议上报道，并提交给《英国放射学杂志》)。1995年，由于非离子二聚体造影剂的延迟不良反应成为亟待解决的医学社会或法医学问题，我们认为应该证实我们先前的结果，并使用非离子二聚体造影剂进行同样的手术。使用新的碘化造影剂，再次评估IgE抗体的产生。除异丙咪醇外，我们还在实验中加入了多种非离子二聚体造影剂(IOTROLAN和IOXANO)。将8周龄BALB/c雄性小鼠随机分为8个实验组，A组为生理盐水B组，B组为DNP-KLH+明矾C组，D组为明矾组，D组为明矾+Iopamidol D组，D组为明矾+Iopamidol E组，DNP-KLM组为明矾+IOtrolan组，F组为明矾+IOtrolan G组，DNP-KLH组为明矾+碘化甘油+碘化甘油。于免疫前、免疫后1周、3周、5周用双抗体夹心酶联免疫吸附试验(EL ISA)法检测血清总IgE含量。结果表明，到目前为止，8个试验组的总IgE均未见明显升高。数据分析仍在继续。

Our investigation during 1993 - 1994, showed a possible adjuvant effect on IgE antibody production in mice. In addition, the adjuvant effect is probably related to the induction immunological reaction of interleukin 4 (reported at contrast media research conference, Turku, Finland, in June 1995 and submitted to the British Journal of Radiology). In 1995, we thought that we should the confirm our previous results mentioned above, and perform a same procedure using nonionic dimeric contrast media, because delayd adverse reaction of nonionic dimeric contrast media became urgent medicosocial or medicolegal problems. The IgE antibody production was again evaluated, using new iodinated contrast media. In addition to iopamidol, we included to varieties of the nonionic dimeric contrast media (iotrolan and iodixanol) in our experiments. BALB/c male mice (8 weeks) are divided into eight experimental groups ; A saline B DNP-KLH + ALUM C DNP-KLH + ALUM + iopamidol D ALUM + iopamidol E DNP-KLM + ALUM + iotrolan F ALUM + iotrolan G DNP-KLH + ALUM + iodixanol H ALUM + iodixanol. Total IgE production was measured at pre-, one week, three weeks, and five weeks after immunization, by using ELISA technique. The results have not shown significant increase of total IgE among the eight experimental groups so far. Data analysis is still being continued.