Fundamentals on Interpretation of Indocyanine Green Fluorescence Angiography

1.为了科学地解读吲哚青绿(ICG)血管造影的各种发现，我们进行了实验工作。ICG在视网膜和脉络膜中定位的组织学证据在大鼠静脉注射ICG微粒后，将其固定在视网膜和脉络膜中，并在红外光学显微镜下观察到ICG的定位。ICG通过脉络膜毛细血管渗漏，然后弥漫地滞留在脉络膜基质中。ICG不能穿透视网膜血管壁和视网膜色素上皮(RPE)。实验性脉络膜梗塞的ICG血管造影术猴在巩膜表面切断睫状后动脉，形成脉络膜梗塞。所有患者均行ICG血管造影、组织病理学及血管铸型准备。ICG血管造影的早期表现为脉络膜循环障碍，晚期表现为增生性RPE。实验性脉络膜新生血管的ICG血管造影术在猴视网膜后极部强激光光凝形成脉络膜新生血管(CNV)。对这些眼进行了ICG血管造影、组织病理学检查和血管铸型准备，ICG血管造影清楚显示了CNV。ICG血管造影显示新生血管被增生性RPE包裹，血管外渗漏停止。这些结果为ICG血管造影的临床解释提供了基础。

1. To read scientifically various findings by Indocyanine green (ICG) angiography, we did experimental work.2. Proof of histological localization of ICG in the retina and choroidIn rats, intravenous by administered ICG particls were fixed in the retina and choroid, and demonstrated localization of ICG by infrared light microscope. ICG leaked through the choriocapillaris, then remained diffusely in the choroidal stroma. ICG did not move through the retinal vessels wall and also the retinal pigment epithelium (RPE).3. ICG angiography in experimental choroidal infarctionIn monkeys, the posterior ciliary arteries were cut on the surface of the sclera, and choroidal infarction were produced. ICG angiography, histopathological study and vascular cast preparation were done on these eyes. Early phase in ICG angiography was coresponded with choroidal circulatory disturbance, and late phase was masked with proliferated RPE.4. ICG angiography in experimental choroidal neovascularizationIn monkeys, choroidal neovascularization (CNV) were produced with intense laser photocoagulation on the posterior pole of the retina. ICG angiography, histopathological study, and vascular cast preparation were done on these eyes.ICG angiography demonstrated CNV clearly. CNV were enclosed with proliferated RPE,then extravascular leakages were ceased in ICG angiography.5. These results showed fundamentals on clinical interpretation of ICG angiography.