Elucidation of the autonomic nervous control of immune organs.

本文观察了机械非伤害性和伤害性机械刺激对麻醉大鼠脾自然杀伤细胞(NK)细胞毒活性和脾血流量的影响。侧胸后肢双侧擦拭体表30min，对脾NK细胞杀伤活性、脾血流量和脾交感神经传出神经活动均无明显影响；双侧后爪皮肤按压刺激30min，脾NK细胞杀伤活性和脾血流量分别降低到67、8和82；夹后爪30min，脾交感传出神经活动增加。电刺激内脏神经后，脾NK细胞的细胞毒活性和脾血流量下降。后爪夹闭引起颈水平脾交感神经或脊髓的抑制。这些结果表明，伤害性后爪夹压后NK细胞的细胞毒活性和脾血流量的代谢是通过脾交感神经介导的一种脊髓上反射反应。另一方面，在中枢神经系统和脊髓损伤的大鼠中，腹部夹30分钟都降低了脾NK细胞的细胞毒活性。因此，腹部刺激通过脊髓反射抑制了NK细胞的活性

The effects of non-noxious and noxious mechanical stimulation of the skin on cytotoxic activity of splenic natural killer (NK) cells and splenic blood flow were examined in anesthetized rats.Bilateral brushing of the body surface between the lateral chest and hindlimb for 30 min did not significantly influence cytotoxic activity of splenic NK cells, splenic blood flow or splenic sympathetic efferent nerve activity.Bilateral pinching stimulation of the skin of the hindpaws for 30 min reduced cytotoxic activity of splenic NK cells and splenic blood flow to 67 <plus-minus> 8 and 82 <plus-minus> 3% of the control values, respectively.Pinching hindpaws for 30 min increased splenic sympathetic efferent nerve activity ton 143 <plus-minus> 15% of control.Electrical stimulation of the splanchnic nerve produced a decrease in the cytotoxic activity of splenic NK cells and splenic blood flow.The hindpaw pinching-induced suppression of the splenic sympathetic nerve or the spinal cord at the cervical level.These results indicate that the supersession of cytotoxic activity of NK cells and splenic blood flow following noxious pinching stimulation of hindpaws is a supra-spinal reflex response mediated via the splenic sympathetic nerve.On the other hand, pinching of abdomen for 30 min reduced cytotoxic activity of splenic NK cells both in the central nervous system intact and spinalized rats.Therefore, the abdominal stimulation suppressed the NK activity via spinal reflex pa