Enzymatic Glycosylation and Phosphatidylation in Organic Solvent Systems.

1.在亲水有机溶剂中脂溶性生理活性化合物的酶糖基化。将含有萜烯醇和纤维素二糖（或乳糖）的混合物在50%乙腈中与杏仁-葡萄糖苷酶（或米曲霉-半乳糖苷酶）孵育后，发现形成了新的长无环萜烯醇衍生物，o - β -糖基法尼醇、o - β -半乳糖法尼醇和o - β -半乳糖基香叶醇。这些糖基化衍生物被分离和鉴定。酚类化合物的糖基化反应-芳香化合物羟基残基的高转糖基化反应。发现嗜热脂肪芽孢杆菌环麦芽糊精葡聚糖转移酶通过转糖基化反应合成苯1- α -葡萄糖苷和0- α - α -葡萄糖基儿茶酚。这些糖苷以结晶形式分离并鉴定。这些糖苷在过氧化物-过氧化氢氧化体系中比苷元稳定得多。水溶性维生素在有机溶剂中的酶促磷脂酰化。发现链霉属磷脂酶D在双相体系中催化1,2-二棕榈酰磷脂酰胆碱（DPPC）的二棕榈酰磷脂酰残基转化为水溶性维生素（硫胺素、泛酸及其相关化合物），分别生成新的脂溶性维生素衍生物（dpp -硫胺素、dpp -泛酸、dpp -硫胺素-二硫丙基、dpp -硫硫素和dpp -泛酸乙醚）。这些新的衍生物被分离和鉴定。

1.Enzymatic glycosylation of fat-solubly physiologically active compounds in a hydrophilic organic solvent.New derivatives of long acyclic terpene alcohols, O-beta-glucosylfarnesol, O-beta-galactosylfarnesol and O-beta-galactosylgeranylgeraniol, were found to be formed, when the mixture containing these terpene alcohols and cellobiose (or lactose) in 50 % acetonitrile was incubated with almond beta-glucosidase (or Aspergillus oryzae beta-galactosidase). These glycosylated derivatives were isolated and identified.Glucosylation of phenolic compounds-A high transglucosylation reaction to hydroxy residue of aromatic compounds.The synthesis of pheny1-alpha-glucoside and 0-alpha-glucosylcatechol via transglucosylation reaction by Bacillus stearothermophilus cyclomaltodextrin glucanotransferase was found. These glucosides were isolated in crystalline form and identified. These glucosides were much more stable than the aglycones in a peroxidase-hydrogen peroxide-oxidizing system.3.Enzymatic phosphatidylation of water-soluble vitamins in organic solvents.Streptomyces sp. phospholipase D was found to be catalyze the transfer reaction of the dipalmitoylphosphatidyl residue from 1,2-dipalmitoy1-3-sn-phosphatidy1-choline (DPPC) to water-soluble vitamins (thiamin, pantothenic acid, and these related compounds) in a biphasic system, to afford novel fat-soluble derivatives of the vitamins (DPP-thiamin, DPP-pantothenic acid, DPP-thiamin-propyldisulfide, DPP-fursulthiamin, and DPP-pantothenyl ethyl ether), respectively. These novel derivatives were isolated and identified.