Studies on in vivo binding ability of anti-nuclear antibodies to epidermal cell nucleus

表皮细胞核染色(ENS)的机制及其与皮肤损害的关系仍存在争议和不清楚。为了更好地了解ENS的病理机制，我们试图寻找自发性自身免疫倾向的ENS模型小鼠。我们的研究结果如下：1.首次用直接免疫荧光法显示了老年MRL/MP-+/+小鼠的表皮细胞核染色。这一阳性染色与受检小鼠肾脏的阳性核染色呈正相关。2.自发性皮肤损害以年龄依赖性的方式出现。光镜下，与SLE易感的mrl/lpr小鼠相比，mrl/n小鼠的淋巴细胞浸润较少。3.表皮细胞核染色与肉眼可见的皮损无关。4.在雌性老年mrl/n小鼠中，表皮核染色与抗RNP抗体呈正相关。

The mechanisms of epidermal nuclear staining (ENS) and its relation to skin lesions are still controversial and unclear. We tried to find spontaneous autoimmune prone model mice with ENS in order to better understand the pathological mechanisms. Our results are as follows ;1.We first demonstrated the epidermal nuclear staining by direct immunofluorescence in aged MRL/Mp-+/+ mice. This positive staining was correlated with the positive nuclear staining of kidney in examined mice.2.Spontaneous skin lesions appeared in the age-dependent manner. Light microscopy showed little lymphocytes infiltration, compared with that of SLE-prone MRL/lpr mic.3.Epidermal nuclear staining was not correlated with macroscopic skin lesions.4.In female aged MRL/n mice, there was the positive association between epidermal nuclear staining and anti-RNP antibodise.These results suggested thatged MRL/n mice provide unique models for investigating the mechanisms of epidermal nuclear staining by direct immunofluorescence technique.