Development of hybrid artificial liver supprt system using lipophilic

研究了增生性结节(HN)包裹肝细胞在人工肝支持系统中作为代谢反应器的解毒作用。化学诱导的增生性结节细胞的NADPH-Cyt.C还原酶活性是正常肝细胞的1.7倍。在有门腔分流的动物上，这种活性更多地是通过酶诱导机制来诱导的。这两种方法的结合有利于获得高性能的药物美洛韦林细胞。但从HN分离肝细胞的步骤并不成功，只能用切碎的HN组织进行进一步研究。包裹的HN细胞通过脂膜表现出跨膜的苯酚葡萄糖醛酸化活性。但活动并未高于预期。形态学观察显示，培养48h时，70-80%的囊化HN细胞呈坏死状。包膜HN细胞低氧灌流可能是HN细胞活性低的原因。建立高效的HN细胞分离方法是将HN细胞应用于人工肝支持的关键。

The utilities of encapsulated hepatocytes from hyperplastic nodule (HN) were studied on their detoxifying abilities as metabolic reactor on artificial liver support system. The cells of chemically induceced hypeplastic nodules exhibited 1.7 fold higher NADPH-Cyt.C Reductase activities than normal hepatocytes. And the activities were more induced on the animal with portacaval shunt through a mechanisum of enzyme induction. Combination of these two methods supposed to be beneficial on getting high performance cells for drug meabolisum. But the step of isolate liver cells from HN was not successful, and minced HN tissue were used for further studies. Encapsulated HN cells exhibited transmembranous phenol glucuronidation activities through lipophilic membranes. But the activity was not higher than expected. On morphological study, 70-80% of cultured encapsulated HN cells exhibited necrotic forms on 48 hours. And low oxigen perfusion in encapsulated HN cells was supposed to be the cause of their low activity. Development of efficient method for the isolation of HN cells was thought to be essential for utilizing the cells on artificial liver support.