Effects of endothelial cell growth factor on endotheliazation of small diameter vascular graft

本项目的目的是评价细胞生长因子对小直径血管移植内皮化的体内影响。为此，我们制作了自制的血管移植物。血管移植物是由聚氨酯制成的，有两层，内多孔层和计算机固体层。将n -己醇壳聚糖凝胶浸渍在这些孔中，在移植物内部形成光滑的凝胶层。在凝胶层中加入酸胚酶生长因子。在体重为2.5 - 2.9 kg的家兔体内研究失败，因为动脉直径相对较小，组织脆弱。该项目的下一步将集中在使用狗等大型动物进行体内研究。

Purpose of this project is to evaluate in vivo effects of cell growth factor on endothelialization within a small diameter vascular graft. For this porpose a house-made vascular graft was fabricated. THe vascular graft was made of polyurethan and had two layrs, inner porous layr and puter solid layr. N-hexanoyl chitosan gel was inpregnated into these pores and smooth gel layr was formed inside the graft. Acid fobroblase growth factor was put into the gel layr. In vivo study using rabbits weighting 2.5 to 2.9 kg was failed because the arota was relatively small in diameter and tissue was fragile. Next step of this project will be focused on in vivo study using larger animal such as dogs.