Chemosensitivity of gynecologic malignancies revealed by disease oriented screening system.

采用MTT法研究子宫内膜癌和卵巢浆液性腺癌的体外化疗敏感性。本研究使用5种已建立的人子宫内膜癌细胞系Ishikawa、HEC-1、HEC-50B、HEC-59和HEC-88，以及3种已建立的卵巢浆液性腺癌细胞系Yoshizaki、Asano和SHIN-3。检测了ADM、THP-ADM、EPI、ACR、DM、Act-D、BLM、PEP、NCS、MMC、VLB、VCR、VDS、ala - c、5-FU、MTX、CPM、CDDP、CBDCA、254-S、MCNU、L-ASP、ETP等23种抗肿瘤药物。将细胞以1 × 10^4/孔接种于微孔板（第0天），培养3天，分别以0、0.01、0.05、0.1、0.2、0.5、1、2、5、10马克/毫升的浓度暴露于每种药物中，1、3、6、12、24、48、72小时（第3天），洗净游离药物，单独培养培养基。MTT法测定酶活性（第7天）。通过存活分数曲线评价各药物的抗肿瘤作用。因此，子宫内膜癌高分化型和低分化型在药物敏感性上存在差异。本研究发现，ADM、THP-ADM、ACR、Act-D、NCS、VLB、CDDP、254-S和ETP对子宫内膜癌有效，ACR、Act-D和CDDP对卵巢浆液性腺癌有效。

Studies are undertaken to examine the chemosensitivity of endometrial cancer and ovarian serous adenocarcinoma in vitro by MTT assay.Five kinds of established human endometrial cancer cell line, Ishikawa, HEC-1, HEC-50B,HEC-59 and HEC-88 and 3 kinds of established ovarian serous adenocarcinoma cell line, Yoshizaki, Asano and SHIN-3 were used in this study.Twenty-three kinds of antitumor agents including ADM,THP-ADM,EPI,ACR,DM,Act-D,BLM,PEP,NCS,MMC,VLB,VCR,VDS,Ara-C,5-FU,MTX,CPM,CDDP,CBDCA,254-S,MCNU,L-ASP,ETP were examined.The cells were inoculated into microplate at 1x10^4/well (day 0) , cultured for 3 days and exposed to each drug at 0,0.01,0.05,0.1,0.2,0.5,1,2,5,10 mug/ml, for 1,3,6,12,24,48,72hs (day 3) , washed free drug and incubate media alone. The enzyme activities were measured by MTT assay (day 7). The antitumor effects of each drug were evaluated from the surviving fraction curves.As a result, there was a difference in drug sensitivity between well differentiated type and poorly differentiated type in endometrial cancer.And this study revealed that ADM,THP-ADM,ACR,Act-D,NCS,VLB,CDDP,254-S and ETP were effective for endometrial cancer and ACR,Act-D and CDDP were effective for ovarian serous adenocarcinoma in vitro.