study of the mechanism and role of fucosylated suger chains abnormal expression associated with uterine corpus cancer onset

本研究的目的是：1)分析与子宫内膜细胞癌变相关的岩藻糖转移酶(FT)的定量和定量变化；2)阐明岩藻糖化糖链在癌细胞转移中的作用。测定了8例正常子宫内膜组织和14例子宫肌体癌组织的α1-2、α1-3、α1-4 FT活性，结果表明，所有癌组织的FT活性均显著升高。培养的SNG-II细胞系来源于高分化子宫体癌，根据识别MSN-1(主要是Lewis^b型糖链)的抗原的存在，将SNG-II细胞分为两个亚系。MSN-1是一种针对子宫癌的岩藻糖化糖链。当测定各亚系的FT活性时，S…的α1-4 FT活性显著增加与几乎不表达MSN-1识别抗原的细胞(SNG-W)相比，表达MSN-1识别抗原的细胞(SNG-S)更多。这些结果有力地表明，岩藻糖化糖链在子宫体癌中的异常表达受岩藻糖转移酶活性的控制，而α1-4 FT活性的变化在这一过程中起着非常重要的作用。2.在研究SNG-S与SNG-W细胞的组织黏附时，采用原位(？)对包埋在美国运通公司的福曼子宫和淋巴结组织标本的黏附实验表明，SNG-W的黏附能力约为SNG-S的10倍，并且这种黏附被MSN-1识别抗原的前体糖链(H型糖链)的单抗明显地以浓度依赖的方式抑制。在一种肺转移模型中，使用裸鼠，通过尾静脉注射和在两种细胞的正常位置移植，获得了相同的结果。这些结果表明，MSN-1识别抗原的前体糖链是岩藻糖化糖链之一，与子宫癌细胞的组织黏附和转移密切相关。这一前体糖链的表达似乎受α1-4 FT活性的控制，这对于阐明癌子宫体高转移水平的机制是一个有趣的结果。较少

The objectives of the present study were 1) analysis of quantitative and quanlitative changes in fucose transferase (FT) associated with canceration of endometrial cells in the uterine corpus, and 2) the role clarification of fucosylated sugar chains in the cancer cells transfer. The following results were obtained from this study in FY 1994.1.When alpha1-2, alpha1-3, alpha1-4 FT activity was measured in eight normal uterine endometrium specimens and 14 cancer tissue from the uterine corpus specimens, it was evident that the FT activity was significantly increased in all the cancer tissue. The cultured cell line SNG-II,derived from well differentiated cancer of the uterine corpus, was divided into two sublines in accordance with the presence of the antigen which recognizes MSN-1 (mainly the Lewis^b type sugar chain), a fucosylated sugar chain specific for uterine corpus cancer. When the FT activity was determined in each subline, the alpha1-4 FT activity was markedly increased in the s … More ells showing strong expression of the MSN-1 recognizing antigen (SNG-S) when compared to the cells showing almost no expression (SNG-W) . These results strongly suggest that abnormal expression of the fucosylated sugar chain in uterine corpus cancer is controlled by fucose transferase activity, and changes in alpha1-4 FT activity plays a very important role in this process. 2.When the tissue adhesion of SNG-S and SNG-W cells was studied, using the in situ (? ) adhesion assay for fuman uterine and lypmh node tissue specimens embedded in AMeX,SNG-W showed about a 10-fold greater adhesion than SNG-S,and it was evident that this adhesion was inhibited in a concentration dependent manner as indicaded by the monoclonal antibody for the precursor sugar chain (H-type sugar chain) of the MSN-1 recognizing antigen. The same results were obtained in a lung metastsis model using intravenous injection into the tail and transplantation at a normal site of both cells, using nude mice. From these findings, it was clear that the precursor sugar chain of the MSN-1 recognizing antigen, one of the fucosylated sugar chains, is closely associated with tissue adhesion and metastasis of uterine corpus cancer cells. The expression of this precursor sugar chain appeared to be controlled by alpha1-4 FT activity, and this is an interesting result for mechanism clarification of the cancer uterine corpus high metastasis levels. Less