Molecular and cell biological investigation about the healing mechanism of gastric mucosal damage.

生长因子在胃损伤修复中的确切细胞机制仍存在争议。我们利用原代培养的兔胃上皮细胞和成纤维细胞，研究了生长因子在胃粘膜修复中的作用。从同一只家兔制备分离的胃上皮细胞和成纤维细胞，并在F-12培养基中培养。接种的上皮细胞（92%为黏液细胞）在48小时内形成完整的单层，成纤维细胞在4周内形成完整的单层。利用旋转硅尖对细胞进行机械剥蚀，形成无细胞面积恒定的创面。使用延时视频系统每12小时测量伤口大小来监测修复过程。BrdU连续染色检测增殖细胞。评估EGF、胰岛素、bFGF、HGF、TGFalpha和PDGF的作用。EGF、胰岛素、HGF、TGFalpha和PDGF加速胃上皮的修复，促进细胞迁移和增殖。bFGF和PDGF均能促进胃间质修复。生长因子靶细胞的差异可能调节胃溃疡愈合，溃疡愈合过程中生长因子网络的完整性是保证溃疡高质量愈合的必要条件。

The precise cellular mechanism of growth factors on gastric wound repair is still controversial. We investigated the role of growth factors on gastric mucosal restoration using primary cultured rabbit gastric epithelial cells and fibroblasts. Isolated rabbit gastric epitherial cells and fibroblasts were prepared from identical rabbit and cultured in F-12 medium. Inoculated epithelial cells (92% mucous cell) formed complete monolayr in 48h and fibroblasts formed complete monolayr in 4 weeks. A wound with cell-freearea of constant size was created by mechanical cell denudation using rotating silicon tip. The restoration process was monitored by measuring wound size every 12 h using a time-lapse video system. The proliferative cells were detected by serial staining for BrdU.Effects of EGF, Insulin, bFGF, HGF, TGFalpha and PDGF were assessed. EGF, Insulin, HGF, TGFalpha and PDGF accelerated the gastric epithelial restoration with the promotion of cell migration and proliferation. bFGF and PDGF accelerated the gastric mesenchymal restoration. The difference of target cells of growth factor might modulate gastric ulcer healing and the integrity of growth factor network during the process of ulcer healing is essential for high quality ulcer healing.