Amphiphilic precision macromolecules to study effects of membrane dynamics (A11#)

在这个提议中，我们的目标是开发一个合成两亲分子的平台，作为生物膜的模仿物和模仿物。通过定制构建块的固相组装，可以获得结构上定义的合成两亲分子，即所谓的两亲精确大分子（AMs），可以分析大范围的结构参数，如亲水头或疏水尾基团的几何形状和组成对膜动力学的影响。与CRC内的合作伙伴一起，将探索AMs改变膜特性，例如通过创建拥挤的膜表面，并研究对膜蛋白的影响，无论是在体外还是在植物体内。

Within this proposal, we aim at developing a platform of synthetic amphiphiles as mimetics and modula-tors of biomembranes. Through solid phase assembly of tailor-made building blocks, structurally defined synthetic amphiphiles, so called amphiphilic precision macromolecules (AMs) can be obtained that allow analyzing the effect of a large range of structural parameters such as the geometry and composition of the hydrophilic head or hydrophobic tail group on membrane dynamics. Together with the collaboration partners within the CRC, AMs will be explored to vary membrane properties e.g. by creating crowded membrane surfaces and study the effects on membrane proteins, both in vitro and in planta.