Hepatic microcirculation and cellular energy level -Visualization of energy dependent processes in vivo-

细胞功能需要能量消耗，因此它依赖于能量的产生。能量守恒过程通常需要氧气和底物，而这些氧气和底物是通过组织中的微循环提供的。因此，肝脏微循环障碍导致肝功能障碍。从肝脏微循环的角度探讨了肝病的发生发展。本研究利用在体荧光显微镜系统观察肝脏能量依赖的生化过程，研究肝脏微循环与细胞能量水平的关系。结果如下：1)大鼠胆汁向胆管的分泌依赖于局部肝组织的氧合作用和器官-组织反射光谱分析的肝脏耗氧量。2)低浓度乙醇增加肝脏O2消耗，高浓度乙醇抑制与…还原有关的氧耗。呼吸链细胞色素n增多，导致肝胆汁分泌下降。胆汁分泌与肝脏能荷(腺嘌呤核苷酸用高效液相色谱测定法)呈线性相关。3)采用带SIT摄像机和视频处理器的在体表观荧光显微镜系统，分析荧光钠从门静脉周围到中心窦和肝细胞以及从肝细胞到胆小管的转运。在正常肝脏中，肝细胞摄取均一，胆小管网络呈多角形，而CC14处理的大鼠则观察到异质性摄取FITC及其分泌，这取决于细胞损伤和微循环障碍。综上所述，利用带有SIT摄像机和视频处理器的体内和体外表观荧光和分光光度显微镜系统，可以实现体内肝脏胆汁分泌等能量依赖性过程的可视化。这项技术有助于可视化肝脏中发生的生理、病理和生化过程。较少

Cellular function needs energy expenditure, so that it depends on energy production. The energy conservation process usually needs the oxygen and the substrates which are supplied through microcirculation in tissues. Thus, the hepatic microcirculatory disturbance results in hepatic dysfunction. The pathogenesis and development of liver disease have been investigated from the aspect of hepatic microcirculation. In this study the relation of hepatic microcirculation with the cellular energy level was studied by visualizing the energy dependent biochemical processes using in vivo epifluorescent microscope system. The findings were as follows: 1)The bile secretion into bile duct in rats depended on regional hepatic tissue oxygenation and hepatic oxygen consumption analyzed by organ-tissue reflectance spectrophotometry. 2)Low concentration of ethanol increased the hepatic 02 consumption, while high concentration of ethanol suppressed the oxygen consumption which was associated with reductio … More n of respiratory chain cytochromes, and resulted in the decline of hepatic bile secretion. The bile secretion was correlated lineally with the hepatic energy charge (the adenine nucleotide being measured with high performance liquid chromatography). 3)In vivo epifluorescent microscope system with SIT camera and video processor was introduced to analyze the transport of fluorescent-Na from periportal to pericentral sinusoids and hepatocytes and from hepatocytes to bile canaliculi. In normal liver a homogeneous uptake by hepatocytes and polygonal appearance of bile canalicular network were observed, while in CC14-treated rats, a heterogeneous uptake of FITC and its secretion were observed, depending on cell damage and also microcirculatory disorders.In conclusion, visualization of energy-dependent processes in the liver in vivo such as bile secretion could be achieved with in vivo and in vitro epifluorescent and spectrophotometric microscope system with SIT camera and video processor. This technique was useful to visualize the physiological, pathological and biochemical processes occurring in the liver. Less