Analysis of Tissue Vasculature Using Near Infrared and Near Suprapurple Endoscopy : Development of the System and its Application to Diagnosis and Treatment of Early Malignant Tumors and other Disorders

使用近红外和近超紫内窥镜分析组织脉管系统：系统的开发及其在早期恶性肿瘤和其他疾病诊断和治疗中的应用

• 批准号: 01870035
• 负责人: KAMADA Takenobu
• 金额: $ 12.16万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01870035/
• 关键词: Key Words; Charge Coupled Device; Image Analysis; Homoglobin; Malignant Tumors; Infrared Light; Suprapurple Light; 近赤外電子内視鏡; 細胞回転; 画像解析システム; 微小血管構築; flowcytometry; 電子内視鏡; 近赤外光; 粘膜ヘモグロビン画像; 粘膜下血管; 胃潰瘍; 胃炎; 微胃癌

Endoscopic detection and treatment of early small cancers are important issues in clinical and social view points. Although convensional endoscopy have relied mainly on human eye, . computerized quantitation has been necessary for early detection of minute cancers in gastrointestinal tractlind bronchus. The investigators have developed computer-assisted electronic endoscopes which are also equ i"!% with near infrared and near suprapurple light source. This endoscopic system is possible to analyze tissue blood hemoglobin distribution at 30, 000 points with a resolution less than 0.01 mm^2/point. The system also detects submucosal vasculature in the gastrointestinal mucosa. Microheterogeneity of gastrointestinal mucosal hemodynamics and subrnucosal vascular responses were investigated in normal subjects and patients with various disorders including peptic ulcer, gastric cancer and various chronic gastritis. The cell proliferation and aniploidity of these subjects were also analyzed by fl … More owcytometry. Furthermore, an algorithm was developed to analyze oxygen saturation of a single capillary for more. precise analysis of tissue hemodynamics.Helicobacter pylori infection has been reported an important epidemiological factor of gastric cancer as well as chronic atrophic gastritis. In an animal experiment, ammonia, which is a product of Helicobacter, was chronically administered to rats. The results revealed that ammonia attenuates mucosal cellular energy metabolism, decreases intracellular mucin content, and increases mucosal cell exfoliation and cell proliferation. Cell population and mucosal histological appearance including gastric vasculature also changed. These changes are quite consistent with those of human gastric mucosa which are shown by the infrared electronic endoscopy. Moreover, another experiment have shown that ammonia is a potent promoter of gastric cancer.Although endoscopic photodynamic therapy, endoscopic resection and endoscopic chemotherapy have been important modalities for treatment of early gastric cancer, these therapy have not distributed widely because of possible remission of the disease. The investigators also examined the effects of various agents on gastrointestinal hemodynamics using the infrared endoscopic system. Ethanol stimulates endothelin-1 and leukotriene releases and causes gastric mucosal blood flow stasis, while a certain antiinflammatory drug decreases mucosal blood hemoglobin content. Possible synergic effect of these agents on endoscopic therapies and development of computer-controlled endoscopic treatment system remain to be examined in the further investigations. Less

早期小癌症的内镜检测和治疗是临床和社会观点的重要问题。尽管传统内窥镜检查主要依赖于人眼，但。计算机定量对于早期检测胃肠道和支气管中的微小癌症是必要的。研究人员开发了计算机辅助电子内窥镜，该内窥镜也具有近红外和近超紫色光源。该内窥镜系统可以分析30, 000个点的组织血液血红蛋白分布，分辨率小于0.01 mm^2/点。该系统还可以检测胃肠粘膜中的粘膜下脉管系统。 研究人员对正常受试者和患有消化性溃疡、胃癌和各种慢性胃炎等各种疾病的患者进行了胃肠粘膜血流动力学和粘膜下血管反应的研究。还通过流式细胞术分析了这些受试者的细胞增殖和单倍体。此外，还开发了一种算法来分析单个毛细血管的氧饱和度。精准分析 据报道，幽门螺杆菌感染是胃癌和慢性萎缩性胃炎的重要流行病学因素。在一项动物实验中，长期给老鼠注射氨（螺杆菌的产物）。结果表明，氨会减弱粘膜细胞的能量代谢，降低细胞内粘蛋白含量，并且 增加粘膜细胞脱落和细胞增殖。细胞群和粘膜组织学外观（包括胃血管系统）也发生了变化。这些变化与红外电子内窥镜显示的人体胃粘膜的变化非常一致。而且，另一项实验表明，氨是胃癌的强效促进剂。虽然内镜光动力疗法、内镜切除术和内镜下 化疗一直是治疗早期胃癌的重要方式，但由于可能缓解疾病，这些疗法尚未广泛推广。研究人员还使用红外内窥镜系统检查了各种药物对胃肠道血流动力学的影响。乙醇刺激内皮素-1和白三烯释放，引起胃粘膜血流瘀滞，同时某些抗炎药物减少 粘膜血中血红蛋白含量。这些药物对内窥镜治疗和计算机控制内窥镜治疗系统的开发可能产生的协同作用仍有待进一步研究。少

项目成果

Ishigami Y.Kawano S.,Nagano K.,Tsuji S.,Hayashi N.,et al.: "Cell proliferation analysis in human gastric ulcer healing at ulcer margin" Gastroenterology. (1991)

Ishigami Y.Kawano S.、Nagano K.、Tsuji S.、Hayashi N.等人：“溃疡边缘人胃溃疡愈合的细胞增殖分析”胃肠病学。

Hayashi N.,Kawano S.,Tsuji S.,Nagano K.,et al.: "Effect of cetraxate hydrochloride on ethanolーinduced gastric mucosal and submucosal hemodynamics in anesthetized dogs." Gastroenterology. (1991)

Hayashi N.、Kawano S.、Tsuji S.、Nagano K. 等人：“盐酸西曲沙酯对麻醉狗乙醇诱导的胃粘膜和粘膜下血流动力学的影响”（胃肠病学）。

Sato N,kawano S,Nagano K: "Gastric mucosal microcirculatory disturbances in portal hypertension." JGastroenterol.Hepatol.4(supp1). 78-80 (1989)

Sato N，kawano S，Nagano K：“门静脉高压症中的胃粘膜微循环障碍。”

Kawano S,Tsujii M,Nagano K,Ogihara T,Tanimura H,Hayashi N,Ito N,et al.: "Different effect of Helicobacter pylori on human gastric antral and body mucosal intracellular mucin." Scand.J.Gastroenterol.25. 997-1003 (1990)

Kawano S，Tsujii M，Nagano K，Ogihara T，Tanimura H，Hayashi N，Ito N，et al.：“幽门螺杆菌对人胃窦和体粘膜细胞内粘蛋白的不同作用。”

Tsuji S, et al.: "Ethanol stimulates immuno-reactive endothelin-1 and-2 release from cultured human umbilical vein endothelial cells." Alcohol Exp Clin Res.

Tsuji S 等人：“乙醇刺激培养的人脐静脉内皮细胞释放免疫反应性内皮素 1 和 2。”