Development of analyzing systems for organ microcirculation and their diagnostic application for minute cancer.

微循环障碍被强调在各种疾病的发生、发展中起重要作用。为了研究三维器官微循环，我们开发了一个在体显微系统，使用SIT相机和图像分析仪。应用双狭缝光度法测定肝窦和胃粘膜毛细血管内红细胞流速。用异硫氰酸荧光素（FITC）检测肝窦经肝细胞向胆小管的转运。在C<Cl_4>-损伤的大鼠肝脏中，RBC流速降低，WBC粘附于窦壁。正常大鼠胃粘膜毛细血管内红细胞流速也存在不均一性。各种药物如吲哚美辛和乙醇引起红细胞流速降低，这取决于药物的浓度。超过20%的乙醇引起出血 ...更多信息 在胃粘膜层，胃粘膜下的血容量和血红蛋白氧合增加，提示胃粘膜下存在动静脉分流。利用电子内窥镜研制了一套真实的实时胃粘膜血流动力学分析系统。结果表明，失血性休克时血流存在明显的微观异质性。这种微异质性与胃溃疡的部位有关。此外，还研制了一种利用红外光源的电子内窥镜对人体胃深层血管进行分析的系统。该系统能清晰地检测到癌区的异常血管结构。总之，本系统是利用带视频处理器的活体显微镜和带红外光的电子视频内窥镜来分析器官微循环的系统。肝窦和胃粘膜毛细血管内血流的微观不均一性在损伤状态和癌变状态下均有表现。少

Microcirculatory disturbance is emphasized to play an important role in the pathogenesis and development of various diseases. In order to investigate the organ microcirculation in three dimensions, we developed an in vivo microscopic system using SIT camera and image analyzer. A method for determining the RBC velocity in hepatic sinusoids and gastric mucosal capillaries was developed with dual slit photometry. Furthermore, fluorescein isothiocyanate(FITC) was used to detect the transport from the sinusoids to the bile canaliculi through hepatocytes. In the C <Cl_4> -damaged rat liver, RBC flow velocity decreased with WBC sticking to the sinusoid walls. FITC transport from sinusoid to bile canaliculi was significantly delayed.In the gastric mucosal capillary, RBC flow velocity also showed the heterogeneity in normal rats. Various agents such as indomethacin and ethanol caused the decrease of RBC flow velocity, depending on the concentration of the drugs. More than 20% ethanol caused hem … More ostasis in the mucosal layer, while in the submucosal layer, the blood volume and hemoglobin oxygenation increased which suggests a presence of arterio-venular shunting in the submucosal layer.A system for analyzing the gastric mucosal hemodynamics in real time, was developed using electronic endoscope. The results showed a marked microheterogeneity in blood flowing under hemorrhagic shock. This microheterogeneity had a relation with the localization of gastric ulcer. Also, a system for analyzing the vasculature in the deeper layer of human stomach was developed using electronic endoscope with infra-red light source. At the cancerous region, the abnormal vasculature pattern was clearly detected by this system.In conclusion, the systems analyzing the organ microcirculation were developed using in vivo microscope with video processors and electronic videoendoscope with infra-red light. The microheterogeneity of blood flowing in hepatic sinusoids and gastric mucosal capillaries were indicated in damaged states and in cancer state. Less