Studies on the structure of capsular polysaccharide produced fron dairy Lactic acid bacteria.

本发明描述了一种开氏乳杆菌。用改良KPL培养基从开菲尔粒中分离到4株乳酸菌，均为产泥、同型发酵、杆状乳酸菌。它们在碳水化合物发酵模式上不同于所有有效描述的乳酸杆菌属同型发酵菌种。从其他六种乳杆菌中获得的脱氧核糖核酸的鸟嘌呤加胞嘧啶含量与新物种的代表性菌株没有显着的相关性。L.通过凝胶过滤、糖组成分析和甲基化分析对开菲尔粒多糖进行了比较研究。开菲尔粒多糖的特性与单发酵乳酸菌相似，但与乳酸菌不同。开菲尔可以得出结论，一种无菌同型发酵乳酸杆菌种是主要负责在粮食中的多糖生产。

Lactobacillus kefiranofaciens sp. nov. is described. Four strains of this species isolated from kefir grains withthe modified KPL medium are slime-forming, homofermentative, rod-shaped lactic acid bacteria. They differ from all the validly descibed homofermentative species of the genus Lactobacillus in their carbohydrate fermentation pattern. Their guanine-plus-cytosine content of deoxyribonucleic acids obtained from six other Lactobacillus species do not show significant relatedness to a representative strain of the new species. The type strain of L. kefiranofaciens sp. nov. is WT-2B(ATCC 43761).Comparative studies of polysaccharides from kefir grains were performed by gel filtration, sugar composition analysis and methylation analysis. The characteristics of the polysaccharides from kefir grains were similar to those of an encasuled monofermentative Lactobacillus species, but different from that of L. kefir. It is concluded that an encapsulad homofermentative Lactobacillus-species is mainly responsible for polysaccharide production in the grains.