The properties and regulations of proteins to form cytoskeletons in higher plants

本文对高等植物细胞骨架元件的性质、功能及其蛋白质组成进行了研究。培养的烟草细胞含有少量微管蛋白样蛋白，其免疫学分类为< α >-微管蛋白。该蛋白诱导微管解聚和抑制聚合。使用抗微管蛋白抗体的免疫荧光研究经常显示微管从细胞核表面向细胞质突出，有时也向细胞核内部突出，就像纺锤体微管形成的起始一样。这一观察结果表明mtoc类物质在核上的定位。事实上，mtoc类活性物质是从高等植物的细胞核中制备出来的。从hifer植物细胞中分离到体外可聚合肌动蛋白。在很短的时间内就能显示出生物化学特性。我们从高等植物细胞中发现了三个新的细胞骨架元素。它们似乎是高等植物中独特的细胞骨架，因此，调节系统…更多的将会感兴趣。50kd蛋白在鸟苷核苷酸存在下聚合成中间丝状结构。该蛋白被证明是碱性蛋白，并与抗微管蛋白抗体交叉反应。单克隆抗体显示50 kd蛋白在间期细胞的细胞核周围和皮层区域形成网络。Phragmoplast也被证明含有这种蛋白质。58kd蛋白在ATP存在的情况下从不溶性部分纯化，该蛋白很容易被ATP的耗尽所溶解。ATP的加入诱导了可逆聚合，形成了直径约为3[5nm的纤丝束。单克隆抗体显示该束分布在细胞核表面，突出于质膜。纤维束可能参与决定细胞质分裂发生的位置和细胞核相对于分裂位置移动的机制。100kd蛋白用不溶性部分的KC1溶解纯化。该蛋白在体外可聚合直径约15nm的双链细丝。这些细胞骨架元件的功能有待进一步研究。少

We have studied about the properties and functions of cytoskeletal elements and its protein compositions in higher plants.Cultured tobacco cells possess minor tubulin-like protein,which is immunologically classified as <alpha>-tubulin. This protein induced the depolymerization and the inhibition of polymerization of microtubules.Immunofluorescent studies using antibodies against tubulin often showed the microtubules protruding from the surface of nuclei to cytoplasm and sometimes to inside of nuclei just like the initiation of spindle microtubules formation.This observation suggested the localization of MTOC-like substances on nuclei.In fact,MTOC-like active substance(s)was prepared from nuclei of higher plants.In vitro polymerizable actin was isolated from hifer plant cells.The biochemical properties would be shown within short time.We found three novel cytoskeletal elements from higher plant cells.They seems to be unique cytoskeletons in higher plants,therefore,the regulation systems … More will be interest.50 kd protein polymerizad intermediate filament-like structures in the presence of guanosine nucleotides.This protein was shown to be basic protein and cross-reacted with antitubulin antibodies.Monoclonal antibodies showed the localization of 50 kd protein around the nuclei and cortical region as network in interphase cells.Phragmoplast was also shown to contain this protein.58 kd protein was purified from insoluble fraction in the presence of ATP,this protein readily solubilized by the depletion of ATP.Addition of ATP induced the reversible polymerization and formed fibrillar bundles of thin filament having a diameter about 3[5 nm.Monoclonal antibodies showed the distribution of this bundles around the surface of nuclei protruding to plasma membrane. Possible involvement of the fibrillar bundles in the mechanism to determine the site at which cytokinesis will occur and to move the nucleus in relation to the division site.100 kd protein was solubilized with KC1 fron insoluble fraction and purified.This protein polymerized double chain filaments haying a diameter about 15 nm in vitro.The function of these cytoskeletal element should be further studied. Less