Structure, Function, and Diseases of Fibronectin and Vitronectin

Vitronectin是1983年发现的一种新的血浆糖蛋白，已被证明具有细胞扩散、凝血、补体等生物活性。我们开发了一种免疫学方法来测量人血液中的Vitronectin浓度。(1)肝功能衰竭患者血浆中Vitronectin浓度显著降低。(2)人血标本中65 kDa和75 kDa多肽的比例不同，可分为三种不同的类型。(3)玻璃体切割血型与目前所检测的疾病无关。2.我们成功地从牛、马、鸡、猪和兔的血浆或血清中分离纯化了Vitronectin，该方法与我们最近开发的用于人血浆的新工艺相同。3.我们克隆了4株产生抗人玻璃体单抗的小鼠杂交瘤细胞株M1、M2、M4和M5。利用这些单抗，推测玻璃连接蛋白的胶原结合区存在于玻璃连接蛋白分子的N-末端半部分。4.我们纯化了几个用甲酸切割的玻璃凝集素片段。其中一个片段似乎对应于胶原结合域。

Vitronectin discovered in 1983 is a new plasma glycoprotein, which has been shown to have biological activities concerning cell spreading, blood coagulation, and complement action.We have investigated the structure and function of vitronectin and the results we have obtained are as follows.1. We have developed the method to measure vitronectin concentration in human blood immunologically. (1) The concentration of vitronectin in human plasma was remarkably low in the person having liver failures. (2) Human blood samples could be classified into three distinct vitronectin types depending on the ratios of 65 kDa and 75 kDa polypeptides of vitronectin. (3) The vitronection blood type was not related with any deseases tested so far. 2. We have succeeded in purification of vitronectin from plasma or sera of bovine, horse, chicken, porcine, and rabbit with the same new procedure developed for human plasma very recently by us. 3. We have cloned 4 mouse hybridoma producing anti-human vitronection monoclomal antibodies, M1, M2, M4, and M5. Using these monoclonal antibodies, collagen-binding domain of vitronectin was suggested to exist on the N-terminal half of vitronectin molecule. 4. We have purified a few vitronectin fragments cleaved with formic acid. One of these fragments seemed to correspond to the collagen-binding domain.