Analysis of ras genes mutations in human hepatic, biliary and pancreatic malignant tumors

ras基因是人类癌症中最常见的致癌基因之一，它由三个家族（H-ras, K-ras,N-ras）组成。这些基因通过发生在密码子12、13或61上的点突变与活性癌基因协调一致。我们采用核苷酸直接测序法、聚合酶链反应法和直接测序法对原发性肝恶性肿瘤和胰腺癌中2个密码子的突变进行了分析。在任何肝细胞癌或肝母细胞瘤中，密码子12,13或61 ras基因周围没有点突变。相比之下，所有胰腺癌都存在点突变。

The ras gene is one of the oncogenes most commonly detected in human cancers, and it consists of three families (H-ras, K-ras,N-ras). There genes are concerted to active oncogenes by point mutations occurring in either codon 12,13,or 61. We analyzed mutation of there codons in primary hepatic malignant tumors and panceatic cancers by a method to directly sequence nucleotides, using polymerase chain reaction and a direct sequencing method. There were no point mutations in any of hepatocellular carcinomas or hepatoblastoma around codon 12,13,or 61 ras genes. In contrast, there were point mutations in the all pancreatic cancers.