Research of experimental pathology on the model for ulcerative colitis - large bowel cancer

1)溃疡性结肠炎-大肠癌模型的建立：对大鼠进行约460天的膳食给予l-羟基蒽醌（HA*）（1%）。29只有效动物中有25只大鼠发生了大肠肿瘤（腺瘤或腺癌）。所有这些有效的大鼠也出现了溃疡性结肠炎患者常见的一些病理改变。它们是隐窝脓肿、黑变、侵蚀或再生上皮。这些结果表明，HA*同时诱导类似溃疡性结肠炎和肿瘤的病理变化。2) HA*给药大鼠增殖上皮细胞的细胞动力学分析：连续观察HA*(1%)给药后4个月、8个月和12个月大鼠的大肠。HA*大鼠大肠壁厚度、隐窝细胞数量、隐窝细胞BrdU标记指标、ODC活性及大肠壁糜烂、隐窝脓肿等变化的外观均明显大于对照组。3) 1,8 -二氢羟基蒽醌（丹赤龙）在大鼠体内的促瘤作用：观察丹赤龙对二甲肼（DMH）诱导的小鼠大肠癌的调节作用。DMH与丹红联合使用的小鼠结肠肿瘤（腺瘤或腺癌）的发生率明显高于单独使用DMH的小鼠。给药小鼠大肠ODC活性明显高于对照组。细胞因子、TNF-a、IL-la在大肠癌发生中的表达：采用RT-PCR法检测细胞因子的表达。细胞因子在结肠肿瘤组织中的表达明显强于非肿瘤组织。给予两种药物或其中任何一种药物的结肠粘膜细胞因子也比对照组大。HA*与醋酸MAM的协同作用可能与HA*产生的炎症条件下表达的细胞因子的生物学效应有关。少

1) Development of the model for ulcerative colitis - large bowel cancer : Dietary administration of l-hydroxyanthraquinone (HA*) (1%) to rats for approximately 460 days was done. Twenty five rats out of 29 effective animals developed large bowel neoplasms (adenoma or adenocarcinoma). All of these effective rats also developed some pathological changes which are often seen in ulcerative colitis patients. They were crypt abscess, melanosis, erosion or regenerative epithels. These results indicate that HA* induces simultaneously pathological changes resembling those appeared in the case of ulcerative colitis and neoplasms.2) Cell kinetic analysis of proliferative epithelial cells in rats given HA* : Sequential observation of the large bowels of rats exposed to HA* (1%) by sacrifice of animals at 4, 8 or 12 months after the start of tye dietary exposure. Thickness, number of cells constituting crypt, labelling indices of BrdU in the crypt cells, ODC activity and appearance of the changes s … More uch as erosion, crypt abscess of the large bowels of rats with HA* were respectively greater than those of control animals. Each of the biomarkers became greater responding to the exposure-time of HA*.3) Tumor-promotive effect of 1, 8-dihydroxianthraquinone (danthron) in the large of mice : Modifying effect of danthron on dimethylhydrazine (DMH)-induced large bowel carcinogenesis in mice was examined. Incidence of colon neoplasms (adenoma or adenocarcinoma) in mice given DMH together with danthron was significantly higher than that of mice exposed to DMH alone. ODC activity of the large bowel of mice given danthron was much greater than that of controls.Expression of cytokines, TNF-a and IL-la in the large bowel carcinogenesis by MAM acetate and HA* : Expression of the cytokines was examined by RT-PCR method. Expression of the cytokines in the colon neoplasms was stronger than in non-neoplastic mucosa. That of cytokines in the colon mucosa given both agents or either of the agents was aloso greater than in controls. The synergistic effects of HA* with MAM acetate might be related to the biological effects of the cytokines expressed in the inflammatory conditioned generated by HA*. Less