Inverted Confocal Laser Scanning Microscope

在这项提案中，我们申请更换使用了10多年的共焦激光扫描显微镜。40多个研究小组使用了该仪器。本文书提供了41篇博士论文、100多篇会议稿件和至少35份同行评议的出版物。仅在过去四年内对出版物进行了系统监测，这意味着出版物的成果可能要高得多。替代仪器将用于不同的科学背景，如医学、自然科学(Anima、Planta)和材料科学。独立于过多的个人极端多才多艺的背景，一些共同的要求与所有提议的研究的大多数有关。该仪器将用于固定样品(细胞、组织、晶体表面)的二维和三维多通道(平均3-4个)图像采集。这台仪器必须提供高分辨率，达到阿贝的限制，但不能超分辨率。发射技术必须能够微调不同发射光谱的检测范围，即使是从同一荧光染料内部在不同条件下改变行为也是如此。一种传统光电倍增管(PMT)和高灵敏度探测器的混合物。激发在可见光光谱中的所有主要标准荧光激发标签都必须是可能的。较大的物理视场，尤其适用于使用较少瓷砖的成像组织和表面，从而减少要应用的缝合。此外，为了获得最佳的三维(z堆叠)成像，必须配备高精度的自动对焦系统。所有这些都需要一个全自动的显微镜工作台。

In this proposal we apply for the replacement of a confocal laser scanning microscope, which now is in use for more than 10 years. More than 40 research groups made use of the instrument. The present instrument served 41 PhD thesises, more than 100 conference contributions and at least 35 peer reviewed publications. Systematic monitoring of publications only was performed within the last four years, which means the outcome of publications is likely to be much higher.The replacement instrument will be used on different scientific backgrounds such as medical, natural science (anima, planta) and material science. Independend from the plethora of individual extremly versatile backgrounds a few common requirements are linked to the majority of all proposed research. The instrument will be used for multi-channel (3 -4 in average) image acquisition of fixed samples (cells, tissue, crystalline surfaces) in 2 and 3 dimensions. The instrument must deliver high-resolution down to Abbe's limitation, but no super resolution. Emission technology must enable to fine tune the detection range of various emission spectra, even from within the same fluorescent dye changing behaviour under different conditions. A mixture of conventional photomultiplying tubes (PMT) and high sensitive detectors. Excitation in the visible spectrum at all major standard fluorescent excitation labels must be possible. Big physical field of view, in particular for imaging tissues and surfaces with less tiles and hence less stitching to apply. Further a high precision auto focus must be in place for best threedimenional (z-stack) imaging. All these requires a fully automated microscope stage.